Cell and Tissue Research

, Volume 324, Issue 2, pp 291–300

Differential expression of caveolin-3 in mouse smooth muscle cells in vivo

  • Hiroshi Kogo
  • Shin-ya Ito
  • Yoshinobu Moritoki
  • Hiroki Kurahashi
  • Toyoshi Fujimoto
Regular Article

DOI: 10.1007/s00441-005-0130-z

Cite this article as:
Kogo, H., Ito, S., Moritoki, Y. et al. Cell Tissue Res (2006) 324: 291. doi:10.1007/s00441-005-0130-z

Abstract

Expression of caveolin-1 and -3 in mouse smooth muscle cells in vivo was examined by immunohistochemistry. Caveolin-1 was detected in almost all smooth muscles examined, except for the pupillary dilator muscle, whereas caveolin-3 was present only in smooth muscles of some specific tissues. In the eye, the pupillary sphincter muscle was intensely positive for caveolin-3, whereas the ciliary muscle and pupillary dilator muscle were negative. In the gastrointestinal tract, caveolin-3 was detected in the inner circular layer, but not in the outer longitudinal layer. Vascular smooth muscle cells of the resistance-sized artery in the uterus and corpus cavernosum were intensely positive for caveolin-3, whereas those of the aorta were only weakly positive and those of the vena cava were negative. Caveolin-3 was also detected in smooth muscle cells of the urinary bladder, ureter, prostatic vas deferens, and seminal vesicle. The different levels of caveolin-3 expression among various smooth muscle tissues were confirmed by Western blot analysis. Even within the same muscle, the relative expression levels of caveolin-1 and -3 were variable among neighboring cells, suggesting distinct fine regulation of expression of these two caveolins. Moreover, even in the same cell, caveolin-1 and -3 showed different distributions. These results indicate that the two caveolins form distinct caveolae in smooth muscles, and that caveolin-1 and -3 serve different functions. Their differential expression may therefore be related to the functional diversity of smooth muscles.

Keywords

Caveolin isoforms Immunohistochemistry Pupillary muscle Gastrointestinal muscle Vascular muscle Mouse (BALB/c) 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hiroshi Kogo
    • 1
    • 2
  • Shin-ya Ito
    • 1
  • Yoshinobu Moritoki
    • 1
  • Hiroki Kurahashi
    • 2
  • Toyoshi Fujimoto
    • 1
  1. 1.Department of Anatomy and Molecular Cell BiologyNagoya University Graduate School of MedicineNagoyaJapan
  2. 2.Division of Molecular Genetics, Institute for Comprehensive Medical ScienceFujita Health UniversityAichiJapan