Cell and Tissue Research

, Volume 322, Issue 2, pp 289–298

In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel

Authors

  • Akiko Yokoyama
    • Section of Orthopaedic Surgery, Graduate SchoolTokyo Medical and Dental University
    • Department of Plastic and Reconstructive SurgeryTokyo Medical and Dental University
    • Section of Orthopaedic Surgery, Graduate SchoolTokyo Medical and Dental University
  • Kyosuke Miyazaki
    • Seikagaku Corporation
  • Shizuko Ichinose
    • Instrumental Analysis Research CenterTokyo Medical and Dental University
  • Yuiro Hata
    • Department of Plastic and Reconstructive SurgeryTokyo Medical and Dental University
  • Takeshi Muneta
    • Section of Orthopaedic Surgery, Graduate SchoolTokyo Medical and Dental University
    • Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and BoneTokyo Medical and Dental University
Regular Article

DOI: 10.1007/s00441-005-0010-6

Cite this article as:
Yokoyama, A., Sekiya, I., Miyazaki, K. et al. Cell Tissue Res (2005) 322: 289. doi:10.1007/s00441-005-0010-6

Abstract

Graft implantation is one of the more popular procedures for repairing cartilage defects; however, sacrifices of the donor site have been an issue. Mesenchymal stem cells (MSCs) are a fascinating source for regenerative medicine because they can be harvested in a less invasive manner and are easily isolated and expanded, with multipotentiality including chondrogenesis. MSCs can be isolated from various adult mesenchymal tissues including synovium. Here, we attempted to form cartilage from the composites of synovium-derived MSCs with collagen gel in vitro. After 21 days of culture, the composites had increased their cartilage matrix, as demonstrated by toluidine blue staining and immunohistochemistry for type II collagen. The composites consisting of 5×107 and 108 cells/ml in gel were richer in proteoglycans than those consisting of lower cell densities. After 1 day, MSCs/gel composites contracted and the diameter decreased by 30%; however, they were stable thereafter. Round cells with short processes producing collagen fibrils showing a similar morphology to that of chondrocytes were seen in the composites by transmission electron microscopy. During composite culture, chondroitin sulfate and mRNA expression for cartilage-related genes increased, demonstrating cartilage maturation. Using an optimized method, we obtained cartilage discs with a diameter of 7 mm and a thickness of 500 μm. Our procedure should thus make it possible to produce a large cartilage matrix in vitro. The tissue engineering of autologous cartilage from the composites of synovium-derived MSCs with collagen gel in vitro for transplantation may be a future alternative to graft implantation for patients with cartilage defects.

Keywords

Mesenchymal stem cells (MSCs)SynoviumCollagen gelIn vitro chondrogenesisTissue engineeringHuman

Copyright information

© Springer-Verlag 2005