Cell and Tissue Research

, Volume 319, Issue 3, pp 429–438

In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells

  • Jinwu Chen
  • Changyong Wang
  • Shuanghong Lü
  • Junzheng Wu
  • Ximin Guo
  • Cuimi Duan
  • Lingzhi Dong
  • Ying Song
  • Junchuan Zhang
  • Dianying Jing
  • Linbo Wu
  • Jiandong Ding
  • Dexue Li
Regular Article

DOI: 10.1007/s00441-004-1025-0

Cite this article as:
Chen, J., Wang, C., Lü, S. et al. Cell Tissue Res (2005) 319: 429. doi:10.1007/s00441-004-1025-0

Abstract

The purpose of this study has been to investigate the possible effects of the normal joint cavity environment on chondrocytic differentiation of bone-marrow-derived mesenchymal stem cells (MSCs). Autologous bone marrow was aspirated from the iliac crest of male sheep. MSCs were purified, expanded, and labeled with the fluorescent dye PKH26. Labeled MSCs were then grown on a three-dimensional porous scaffold of poly (L-lactic-co-glycolic acid) in vitro and implanted into the joint cavity by a surgical procedure. At 4 or 8 weeks after implantation, the implants were removed for histochemical and immunohistochemical analysis. The cells labeled with red fluorescent PKH26 in the implants expressed type II collagen and synthesized sulfated proteoglycans. However, the osteoblast-specific marker, osteocalcin, was not detected by immunohistochemistry indicating that the implanted MSCs had not differentiated into osteoblasts by being directly exposed to the normal joint cavity. To investigate the possible factors involved in chondrocytic differentiation of MSCs further, we co-cultured sheep MSCs with the main components of the normal joint cavity, viz., synovial fluid or synovial cells, in vitro. After 1 or 2 weeks of co-culture, the MSCs in both co-culture systems expressed markers of chondrogenesis. These results suggest that synovial fluid and synovium from normal joint cavity are important for the chondrocytic differentiation of adult bone-marrow-derived MSCs.

Keywords

Mesenchymal stem cellChondrogenesisMicroenvironmentTissue engineeringIn vivo differentiationSheep

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jinwu Chen
    • 1
    • 2
  • Changyong Wang
    • 1
  • Shuanghong Lü
    • 1
  • Junzheng Wu
    • 2
  • Ximin Guo
    • 1
  • Cuimi Duan
    • 1
  • Lingzhi Dong
    • 1
  • Ying Song
    • 3
  • Junchuan Zhang
    • 4
  • Dianying Jing
    • 4
  • Linbo Wu
    • 4
  • Jiandong Ding
    • 4
  • Dexue Li
    • 1
  1. 1.Beijing Institute of Basic Medical SciencesBeijingPeople’s Republic of China
  2. 2.The Stomatological SchoolThe Fourth Military Medical UniversityXi’anPeople’s Republic of China
  3. 3.Organ Recovery SystemsPort City CenterCharlestonUSA
  4. 4.Department of Macromolecular Science, Key Laboratory of Molecular Engineering of Polymers of Ministry of EducationFudan UniversityShanghaiPeople’s Republic of China