Regular Article

Cell and Tissue Research

, Volume 316, Issue 2, pp 141-153

First online:

Custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet

  • Kohei TsuchiyaAffiliated withDepartment of Reproductive Biology and Pathology, National Research Institute for Child and Health DevelopmentDepartment of Pathology, Keio University School of MedicineDepartment of Orthopedic Surgery, Asahikawa Medical CollegeBiomaterials Center, National Institute for Materials Science
  • , Taisuke MoriAffiliated withDepartment of Reproductive Biology and Pathology, National Research Institute for Child and Health DevelopmentDepartment of Pathology, Keio University School of Medicine
  • , Guoping ChenAffiliated withBiomaterials Center, National Institute for Materials ScienceTissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology
  • , Takashi UshidaAffiliated withTissue Engineering Research Center, National Institute of Advanced Industrial Science and TechnologyCenter for Disease Biology and Integrative Medicine, School of Medicine, University of Tokyo
  • , Tetsuya TateishiAffiliated withBiomaterials Center, National Institute for Materials Science
  • , Takeo MatsunoAffiliated withDepartment of Orthopedic Surgery, Asahikawa Medical College
  • , Michiie SakamotoAffiliated withDepartment of Pathology, Keio University School of Medicine
  • , Akihiro UmezawaAffiliated withDepartment of Reproductive Biology and Pathology, National Research Institute for Child and Health Development Email author 

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Abstract

New bone for the repair or the restoration of the function of traumatized, damaged, or lost bone is a major clinical need, and bone tissue engineering has been heralded as an alternative strategy for regenerating bone. A novel web-like structured biodegradable hybrid sheet has been developed for bone tissue engineering by preparing knitted poly(DL-lactic-co-glycolic acid) sheets (PLGA sheets) with collagen microsponges in their openings. The PLGA skeleton facilitates the formation of the hybrid sheets into desired shapes, and the collagen microsponges in the pores of the PLGA sheet promote cell adhesion and uniform cell distribution throughout the sheet. A large number of osteoblasts established from marrow stroma adhere to the scaffolds and generate the desired-shaped bone in combination with these novel sheets. These results indicate that the web-like structured novel sheet shows promise for use as a tool for custom-shaped bone regeneration in basic research on osteogenesis and for the development of therapeutic applications.

Keywords

Bone regeneration Tissue engineering Scaffold Marrow stroma Polymer KUSA-A1 cells