Regular Article

Cell and Tissue Research

, Volume 315, Issue 2, pp 157-166

First online:

Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts

  • Stefan HammAffiliated withMax Planck Institute for Physiological and Clinical ResearchWyeth Vaccine Research
  • , Bénédicte DehouckAffiliated withMax Planck Institute for Physiological and Clinical ResearchUnité mixte Institut Pasteur de Lille-Université d’Artois, U.A. 2465-Faculté des Sciences Jean Perrin
  • , Jörg KrausAffiliated withMax Planck Institute for Physiological and Clinical ResearchMax Planck Institute for Vascular Biology
  • , Karen Wolburg-BuchholzAffiliated withMax Planck Institute for Physiological and Clinical ResearchInstitute for Pathology
  • , Hartwig WolburgAffiliated withInstitute for Pathology
  • , Werner Risau
  • , Roméo CecchelliAffiliated withUnité mixte Institut Pasteur de Lille-Université d’Artois, U.A. 2465-Faculté des Sciences Jean Perrin
  • , Britta EngelhardtAffiliated withMax Planck Institute for Physiological and Clinical ResearchMax Planck Institute for Vascular BiologyTheodor Kocher Institute, University of Bern Email author 
  • , Marie-Pierre DehouckAffiliated withUnité mixte Institut Pasteur de Lille-Université d’Artois, U.A. 2465-Faculté des Sciences Jean Perrin

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Abstract

In the central nervous system (CNS) complex endothelial tight junctions (TJs) form a restrictive paracellular diffusion barrier, the blood-brain barrier (BBB). Pathogenic changes within the CNS are frequently accompanied by the loss of BBB properties, resulting in brain edema. In order to investigate whether BBB leakiness can be monitored by a loss of TJ proteins from cellular borders, we used an in vitro BBB model where brain endothelial cells in co-culture with astrocytes form a tight permeability barrier for 3H-inulin and 14C-sucrose. Removal of astrocytes from the co-culture resulted in an increased permeability to small tracers across the brain endothelial cell monolayer and an opening of the TJs to horseradish peroxidase as detected by electron microscopy. Strikingly, opening of the endothelial TJs was not accompanied by any visible change in the molecular composition of endothelial TJs as junctional localization of the TJ-associated proteins claudin-3, claudin-5, occludin, ZO-1 or ZO-2 or the adherens junction-associated proteins β-catenin or p120cas did not change. Thus, opening of BBB TJs is not readily accompanied by the complete loss of the junctional localization of TJ proteins.

Keywords

Claudins Occludin Tight junction Edema formation Blood-brain barrier Cell culture (bovine, rat)