Cell and Tissue Research

, Volume 315, Issue 2, pp 197–201

Localization of Aa-nat mRNA in the rat retina by fluorescence in situ hybridization and laser capture microdissection

  • Cuimei Liu
  • Chiaki Fukuhara
  • James H. WesselIII
  • P. Michael Iuvone
  • Gianluca Tosini
Regular Article

DOI: 10.1007/s00441-003-0822-1

Cite this article as:
Liu, C., Fukuhara, C., Wessel, J.H. et al. Cell Tissue Res (2004) 315: 197. doi:10.1007/s00441-003-0822-1

Abstract

Arylalkylamine N-acetyltransferase (AA-NAT) is the key regulatory enzyme in the melatonin biosynthetic pathway. Previous investigations have reported that Aa-nat mRNA in rat is only detected in a sub-population of photoreceptor cells that resemble cones in shape and size. In the present study, we investigated Aa-nat expression in the rat retina by using in situ hybridization and laser capture microdissection combined with the reverse transcription/polymerase chain reaction technique. Our results demonstrate that, contrary to previous reports, Aa-nat transcripts are present not only in the photoreceptor cells, but also in the inner nuclear layer and in the ganglion cell layer. However, the rhythmic expression of Aa-nat mRNA was observed only in photoreceptor cells.

Keywords

RetinaMelatoninCircadian rhythmAa-natPhotoreceptorsGanglion cellsRat (Wistar, adult male)

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Cuimei Liu
    • 1
  • Chiaki Fukuhara
    • 1
  • James H. WesselIII
    • 2
  • P. Michael Iuvone
    • 2
  • Gianluca Tosini
    • 1
  1. 1.Neuroscience Institute and NSF Center for Behavioral NeuroscienceMorehouse School of MedicineAtlantaUSA
  2. 2.Department of PharmacologyEmory University School of MedicineAtlantaUSA