Original investigation

Human Genetics

, Volume 102, Issue 6, pp 675-680

Five novel missense mutations of the Lewis gene (FUT3) in African (Xhosa) and Caucasian populations in South Africa

  • H. PangAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp
  • , Yuhua LiuAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp
  • , Yoshiro KodaAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp
  • , Mikiko SoejimaAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp
  • , Jingtao JiaAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp
  • , Terry SchlaphoffAffiliated withLaboratory for Tissue Immunology, Department of Immunology, Medical School, Cape Town 7935, South Africa
  • , Ernette D. du ToitAffiliated withLaboratory for Tissue Immunology, Department of Immunology, Medical School, Cape Town 7935, South Africa
  • , H. KimuraAffiliated withDivision of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan Fax: +81 942 31 7700, e-mail: hkimura@med.kurume-u.ac.jp

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Abstract

Five novel missense mutations, viz., C304 A, T370 G, G484 A, G667 A, and G808 A, in the Lewis gene (FUT3) were detected in African (Xhosa) and Caucasian individuals in South Africa. These single base substitutions may result in changes in amino acid residues from Gln102 to Lys in the 304 mutation, Ser124 to Ala in the 370 mutation, Asp162 to Asn in the 484 mutation, Gly223 to Arg in the 667 mutation, and Val270 to Met in the 808 mutation. Out of the five novel mutations identified in this investigation, four new alleles (le 484,667 , le 484,667,808 , Le 304 , and Le 370 ) were determined in the Xhosa population and two new alleles (le 202,314,484 and Le 304 ) in the Caucasian population. The determination of α(1,3/1,4)fucosyltransferase activity, after transfection of plasmids containing the new alleles into COS7 cells, suggested that alleles le 484,667 and le 484,667,808 encoded an inactive enzyme, and that alleles Le 304 and Le 370 encoded a functional enzyme. In addition, we also examined the incidence of five common alleles, Le 59 , le 59,508 , le 59,1067 , le 202,314 , and le 1067 in two populations by the polymerase chain reaction/restriction fragment length polymorphism method and compared differences in the allele frequencies of FUT3 among three ethnic groups (Orientals, Africans, and Caucasians).