Original investigation

Human Genetics

, Volume 102, Issue 4, pp 397-402

First online:

Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals

  • Hiroshi IwamaAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , K. OhyashikiAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Junko H. OhyashikiAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Shigifumi HayashiAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Naoyuki YahataAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Keiko AndoAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Keisuke ToyamaAffiliated withThe First Department of Internal Medicine, Tokyo Medical College, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo 160, Japan Tel.: +81-3-3342-6111 ext. 5894, Fax: +81-3-5381-6651, e-mail: ohyashik@rr.iij4u.or.jp
  • , Akinori HoshikaAffiliated withDepartment of Pediatrics, Tokyo Medical College, Tokyo 160, Japan
  • , Masaru TakasakiAffiliated withDepartment of Geriatric Medicine, Tokyo Medical College, Tokyo 160, Japan
    • , Mayumi MoriAffiliated withDepartment of Internal Medicine, Tokyo Metropolitan Geriatric Hospital, Japan
    • , Jerry W. ShayAffiliated withDepartment of Cell Biology and Neuroscience, The University of Texas Southwestern Medical Center, Dallas, TX 75235-9039, USA

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Abstract

The telomerase activity and length of telomeres of peripheral blood mononuclear cells obtained from 124 healthy individuals aged 4–95years was measured. Telomerase activity level was semiquantitatively assessed by a fluorescent-telomeric repeat amplification protocol (fluorescent-TRAP) using an internal telomerase assay standard, fluorescent primers and an automated laser fluorescent DNA sequencer. Telomeric length, measured by assay of terminal restriction fragments (TRFs), was determined in HinfI-digested DNA by Southern blot analysis using a (TTAGGG)4 probe. TRF length was determined in 80 individuals and age-related progressive reduction of size was observed. TRF length in peripheral blood mononuclear cells obtained from normal individuals (aged 4–39years) decreased by approximately 84bp per year, while in individuals aged 40years it decreased by 41bp per year. In contrast, telomerase activity showed an apparent biphasic pattern with aging. Individuals aged 4–39years showed a progressive decrease in telomerase activity, whereas 65% of those aged 40years showed relatively stable but very low telomerase activity, and the remaining individuals aged 40years had no detectable telomerase activity. These data obtained from normal individuals might in the future be of value to help risk stratify and manage the care of patients with leukemia.