Human Genetics

, Volume 99, Issue 4, pp 550–557

nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13

  • Laurence Milon
  • Marie-Françoise Rousseau-Merck
  • Annie Munier
  • Muriel Erent
  • Ioan Lascu
  • Jacqueline Capeau
  • M.-L. Lacombe
Rapid communication

DOI: 10.1007/s004390050405

Cite this article as:
Milon, L., Rousseau-Merck, M., Munier, A. et al. Hum Genet (1997) 99: 550. doi:10.1007/s004390050405

Abstract

A novel human nm23/nucleoside diphosphate (NDP) kinase gene, called nm23-H4, was identified by screening a human stomach cDNA library with a probe generated by amplification by reverse transcription-polymerase chain reaction. The primers were designed from publicly available database cDNA sequences selected according to their homology to the human nm23-H1 putative metastasis suppressor gene. The full-length cDNA sequence predicts a 187 amino acid protein possessing the region homologous to NDP kinases with all residues crucial for nucleotide binding and catalysis, strongly suggesting that Nm23-H4 possesses NDP kinase activity. It shares 56, 55 and 60% identity with Nm23-H1, Nm23-H2 and DR-Nm23, respectively, the other human Nm23 proteins isolated so far. Compared with these proteins, Nm23-H4 contains an additional NH2-terminal region that is rich in positively charged residues and could indicate routing to mitochondria. The nm23-H4 gene has been localised to human chromosomal band 16p13.3. The corresponding 1.2 kb mRNA is widely distributed and expressed in a tissue-dependent manner, being found at very high levels in prostate, heart, liver, small intestine and skeletal muscle tissues and in low amounts in the brain and in blood leucocytes. Nm23-H4 naturally possesses the Pro-Ser substitution equivalent to the K-pn mutation (P97S) of Drosophila.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Laurence Milon
    • 1
  • Marie-Françoise Rousseau-Merck
    • 2
  • Annie Munier
    • 1
  • Muriel Erent
    • 3
  • Ioan Lascu
    • 3
  • Jacqueline Capeau
    • 1
  • M.-L. Lacombe
    • 1
  1. 1.INSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France Tel.: +33-1-40011355; Fax: +33-1-40011499 e-mail: lacombe@ccr.jussieu.frFR
  2. 2.INSERM U 301, IGM, Hôpital Saint-Louis, Paris, FranceFR
  3. 3.Université de Bordeaux 2, IBGC-CNRS, Bordeaux, FranceFR