Rapid communication

Human Genetics

, Volume 99, Issue 4, pp 550-557

First online:

nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13

  • Laurence MilonAffiliated withINSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France Tel.: +33-1-40011355; Fax: +33-1-40011499 e-mail: lacombe@ccr.jussieu.fr
  • , Marie-Françoise Rousseau-MerckAffiliated withINSERM U 301, IGM, Hôpital Saint-Louis, Paris, France
  • , Annie MunierAffiliated withINSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France Tel.: +33-1-40011355; Fax: +33-1-40011499 e-mail: lacombe@ccr.jussieu.fr
  • , Muriel ErentAffiliated withUniversité de Bordeaux 2, IBGC-CNRS, Bordeaux, France
  • , Ioan LascuAffiliated withUniversité de Bordeaux 2, IBGC-CNRS, Bordeaux, France
  • , Jacqueline CapeauAffiliated withINSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France Tel.: +33-1-40011355; Fax: +33-1-40011499 e-mail: lacombe@ccr.jussieu.fr
  • , M.-L. LacombeAffiliated withINSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France Tel.: +33-1-40011355; Fax: +33-1-40011499 e-mail: lacombe@ccr.jussieu.fr

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A novel human nm23/nucleoside diphosphate (NDP) kinase gene, called nm23-H4, was identified by screening a human stomach cDNA library with a probe generated by amplification by reverse transcription-polymerase chain reaction. The primers were designed from publicly available database cDNA sequences selected according to their homology to the human nm23-H1 putative metastasis suppressor gene. The full-length cDNA sequence predicts a 187 amino acid protein possessing the region homologous to NDP kinases with all residues crucial for nucleotide binding and catalysis, strongly suggesting that Nm23-H4 possesses NDP kinase activity. It shares 56, 55 and 60% identity with Nm23-H1, Nm23-H2 and DR-Nm23, respectively, the other human Nm23 proteins isolated so far. Compared with these proteins, Nm23-H4 contains an additional NH2-terminal region that is rich in positively charged residues and could indicate routing to mitochondria. The nm23-H4 gene has been localised to human chromosomal band 16p13.3. The corresponding 1.2 kb mRNA is widely distributed and expressed in a tissue-dependent manner, being found at very high levels in prostate, heart, liver, small intestine and skeletal muscle tissues and in low amounts in the brain and in blood leucocytes. Nm23-H4 naturally possesses the Pro-Ser substitution equivalent to the K-pn mutation (P97S) of Drosophila.