Original investigation

Human Genetics

, Volume 98, Issue 2, pp 162-166

Fetal cells in maternal blood: recovery by charge flow separation

  • S. S. WachtelAffiliated withDepartment of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USA
  • , David SammonsAffiliated withBioSeparations Inc., Tucson AZ 85719, USA
  • , Michael ManleyAffiliated withBioSeparations Inc., Tucson AZ 85719, USA
  • , Gwendolyn WachtelAffiliated withDepartment of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USA
  • , Garland TwittyAffiliated withBioSeparations Inc., Tucson AZ 85719, USA
  • , Joseph UtermohlenAffiliated withBioSeparations Inc., Tucson AZ 85719, USA
  • , Owen P. PhillipsAffiliated withDepartment of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USA
  • , Lee P. ShulmanAffiliated withDepartment of Obstetrics and Gynecology, University of Tennessee, Memphis TN 38103, USA
  • , Douglas J. TaronAffiliated withVysis Inc., Downers Grove IL 60515, USA
    • , U. R. MüllerAffiliated withVysis Inc., Downers Grove IL 60515, USA
    • , Peter KoeppenAffiliated withVysis Inc., Downers Grove IL 60515, USA
    • , Teresa M. RuffaloAffiliated withVysis Inc., Downers Grove IL 60515, USA
    • , Karen AddisAffiliated withCopperstate Ob-Gyn, Tucson AZ 85712, USA
    • , Richard PorrecoAffiliated withRocky Mountain Perinatal Associates, Denver CO 80218, USA
    • , Joyce Murata-CollinsAffiliated withThe Children’s Hospital and The University of Colorado School of Medicine, Denver CO 80218, USA
    • , Natalie B. ParkerAffiliated withThe Children’s Hospital and The University of Colorado School of Medicine, Denver CO 80218, USA
    • , Loris McGavranAffiliated withThe Children’s Hospital and The University of Colorado School of Medicine, Denver CO 80218, USA

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Abstract

Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.