Human Genetics

, Volume 120, Issue 4, pp 461–469

A molecular mechanism for the differential regulation of TGF-β1 expression due to the common SNP −509C-T (c. −1347C > T)

  • Riddhish Shah
  • Carolyn K. Hurley
  • Phillip E. Posch
Original Investigation

DOI: 10.1007/s00439-006-0194-1

Cite this article as:
Shah, R., Hurley, C.K. & Posch, P.E. Hum Genet (2006) 120: 461. doi:10.1007/s00439-006-0194-1

Abstract

Transforming growth factor β1 (TGF-β1) levels influence many cellular, immunologic and pathologic processes. Activator protein 1 (AP1) and hypoxia are key regulators of TGF-β1 expression levels. The common TGFB1 promoter SNP c.−1347C > T (−509C-T, rs1800469) has been linked to a nearly twofold difference in plasma levels among individuals and with risk, progression, and outcome of numerous diseases. We demonstrate exclusive in vitro and in vivo recruitment of AP1 containing JunD to −1347C. This study also is the first to demonstrate hypoxia inducible factor 1 (HIF-1) binding to the TGFB1 promoter. HIF-1 was found to associate with both −1347C and −1347T and compete with AP1 for binding to −1347C. Reporter constructs demonstrate that expression differences between −1347C and −1347T are due to selective AP1 recruitment to the TGFB1 promoter. As AP1 is known to down-regulate transcription of other genes, we suggest that the molecular mechanism for the difference in TGF-β1 plasma levels linked to −1347 is due to transcriptional suppression by AP1 binding to −1347C. These data should aid in our understanding of the association of the −1347 SNP with the pathogenesis of certain TGF-β1-related diseases.

Abbreviations

SNP

Single nucleotide polymorphism

IHWS

International Histocompatibility Workshop

YY1

Yin-yang 1

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Riddhish Shah
    • 1
  • Carolyn K. Hurley
    • 1
  • Phillip E. Posch
    • 2
  1. 1.Department of Oncology Georgetown University Medical CenterWashingtonUSA
  2. 2.Department of Microbiology and Immunology, E408 New Research BuildingGeorgetown University Medical CenterWashingtonUSA