Human Genetics

, Volume 113, Issue 4, pp 307–310

Evaluation of bidirectional transfer of plasma DNA through placenta

  • Akihiko Sekizawa
  • Kaori Yokokawa
  • Yumi Sugito
  • Mariko Iwasaki
  • Yasuo Yukimoto
  • Kiyotake Ichizuka
  • Hiroshi Saito
  • Takashi Okai
Original Investigation

DOI: 10.1007/s00439-003-0987-4

Cite this article as:
Sekizawa, A., Yokokawa, K., Sugito, Y. et al. Hum Genet (2003) 113: 307. doi:10.1007/s00439-003-0987-4

Abstract

To clarify the origin of cell-free fetal DNA in maternal plasma, we analyzed bidirectional transfer of plasma DNA between fetus and mother. We analyzed maternal and fetal plasma DNA obtained from 15 pregnant women at the time of Cesarean section. The subjects were five patients with preeclampsia and 10 gestational-age-matched normal controls. DNA was extracted from 1.5-ml plasma samples and the cellular fraction of maternal and umbilical blood. Seven polymorphic marker genes were analyzed. The relative concentration of fetal DNA in maternal plasma and maternal DNA in cord blood were evaluated. The relative concentration of maternal DNA in fetal circulation (median, 0.9%; range, 0.2–8.4%) was significantly lower than that of fetal DNA in maternal blood (14.3%, 2.3–64%), with P=0.007. The relative concentration of maternal DNA in fetal blood was not affected by preeclampsia. These findings indicate that cell-free DNA is unequally transferred through the placenta. The structural characteristics of the placenta suggest that the majority of cell-free fetal DNA in maternal plasma is derived from villous trophoblasts.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Akihiko Sekizawa
    • 1
  • Kaori Yokokawa
    • 1
  • Yumi Sugito
    • 1
  • Mariko Iwasaki
    • 1
  • Yasuo Yukimoto
    • 1
  • Kiyotake Ichizuka
    • 1
  • Hiroshi Saito
    • 1
  • Takashi Okai
    • 1
  1. 1.Department of Obstetrics and GynecologyShowa University School of MedicineTokyo Japan