Human Genetics

, Volume 113, Issue 4, pp 307–310

Evaluation of bidirectional transfer of plasma DNA through placenta

Authors

    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Kaori Yokokawa
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Yumi Sugito
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Mariko Iwasaki
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Yasuo Yukimoto
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Kiyotake Ichizuka
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Hiroshi Saito
    • Department of Obstetrics and GynecologyShowa University School of Medicine
  • Takashi Okai
    • Department of Obstetrics and GynecologyShowa University School of Medicine
Original Investigation

DOI: 10.1007/s00439-003-0987-4

Cite this article as:
Sekizawa, A., Yokokawa, K., Sugito, Y. et al. Hum Genet (2003) 113: 307. doi:10.1007/s00439-003-0987-4

Abstract

To clarify the origin of cell-free fetal DNA in maternal plasma, we analyzed bidirectional transfer of plasma DNA between fetus and mother. We analyzed maternal and fetal plasma DNA obtained from 15 pregnant women at the time of Cesarean section. The subjects were five patients with preeclampsia and 10 gestational-age-matched normal controls. DNA was extracted from 1.5-ml plasma samples and the cellular fraction of maternal and umbilical blood. Seven polymorphic marker genes were analyzed. The relative concentration of fetal DNA in maternal plasma and maternal DNA in cord blood were evaluated. The relative concentration of maternal DNA in fetal circulation (median, 0.9%; range, 0.2–8.4%) was significantly lower than that of fetal DNA in maternal blood (14.3%, 2.3–64%), with P=0.007. The relative concentration of maternal DNA in fetal blood was not affected by preeclampsia. These findings indicate that cell-free DNA is unequally transferred through the placenta. The structural characteristics of the placenta suggest that the majority of cell-free fetal DNA in maternal plasma is derived from villous trophoblasts.

Copyright information

© Springer-Verlag 2003