Molecular Genetics and Genomics

, Volume 266, Issue 2, pp 318–325

Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea

  •  K. Ikeda
  •  H. Nakayashiki
  •  M. Takagi
  •  Y. Tosa
  •  S. Mayama
Original Paper

DOI: 10.1007/s004380100560

Cite this article as:
Ikeda, K., Nakayashiki, H., Takagi, M. et al. Mol Gen Genomics (2001) 266: 318. doi:10.1007/s004380100560

Abstract.

MAGGY is a gypsy-like retrotransposon isolated from the plant pathogenic fungus Magnaporthe grisea. The ability of various stresses to activate MAGGY was tested in the original and in a heterologous host (Colletotrichum lagenarium), using β-glucuronidase (GUS) as a reporter. The MAGGY promoter was activated in M. grisea by either heat shock, copper sulfate, or oxidative stress, but not by the antifungal substance p-coumaric acid. Transcriptional up-regulation of MAGGY RNA was also observed following heat shock and oxidative stress. The MAGGY promoter remained responsive to the above-mentioned stresses when transformed into a M. grisea isolate that had no endogenous MAGGY elements. In C. lagenarium, however, the MAGGY promoter showed only basal expression of GUS and no further up-regulation was induced by any of the stress treatments, suggesting that the stress-responding cis-element(s) in the MAGGY promoter is not functional in a wider range of fungi. The relationship between the activation of MAGGY by stress and phenotypic diversification in M. grisea, including variations in pathogenicity, is discussed.

Activation MAGGY Magnaporthe grisea Retrotransposon Stress 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  K. Ikeda
    • 1
  •  H. Nakayashiki
    • 1
  •  M. Takagi
    • 1
  •  Y. Tosa
    • 1
  •  S. Mayama
    • 1
  1. 1.Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan

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