Molecular Genetics and Genomics

, Volume 266, Issue 4, pp 556–563

DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid

Authors

  •  P. Daborn
    • Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK
  •  S. Boundy
    • Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK
  •  J. Yen
    • Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK
  •  B. Pittendrigh
    • Department of Entomology, Purdue University, West Lafayette, IN 47907, USA
  •  R. ffrench-Constant
    • Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK
Original Paper

DOI: 10.1007/s004380100531

Cite this article as:
Daborn, P., Boundy, S., Yen, J. et al. Mol Gen Genomics (2001) 266: 556. doi:10.1007/s004380100531

Abstract.

Mutagenesis can be used as a means of predicting likely mechanisms of resistance to novel classes of insecticides. We used chemical mutagenesis in Drosophila to screen for mutants that had become resistant to imidacloprid, a neonicotinoid insecticide. Here we report the isolation of two new dominant imidacloprid-resistant mutants. By recombinational mapping we show that these map to the same location as Rst(2)DDT. Furthermore, we show that pre-existing Rst(2)DDT alleles in turn confer cross-resistance to imidacloprid. In order to localize the Rst(2)DDT gene more precisely, we mapped resistance to both DDT and imidacloprid with respect to P-element markers whose genomic location is known. By screening for recombinants between these P-elements and resistance we localized the gene between 48D5–6 and 48F3–6 on the polytene chromosome map. The genomic sequence in this interval shows a cluster of cytochrome P450 genes, one of which, Cyp6g1, is over-expressed in all resistant strains examined. We are now testing the hypothesis that resistance to both compounds is associated with over-expression of this P450 gene.

Insecticide resistance DDT Imidacloprid Cytochrome P450 Drosophila melanogaster

Copyright information

© Springer-Verlag 2001