Molecular and General Genetics MGG

, Volume 263, Issue 2, pp 328–334

An AFLP marker that differentiates biotypes of the Asian rice gall midge (Orseolia oryzae, Wood-Mason) is sex-linked and also linked to avirulence

  • S. K. Behura
  • S. Nair
  • S. C. Sahu
  • M. Mohan
ORIGINAL PAPER

DOI: 10.1007/s004380051175

Cite this article as:
Behura, S., Nair, S., Sahu, S. et al. Mol Gen Genet (2000) 263: 328. doi:10.1007/s004380051175

Abstract

In an attempt to identify a specific marker for biotype 2 of the Asian rice gall midge (Orseolia oryzae, Wood-Mason), we used AFLP (amplified fragment length polymorphism) fingerprinting. We identified an AFLP marker that is specifically amplified in biotypes 1, 2 and 5 of the rice gall midge, but not in biotype 4. Biotypes 1, 2 and 5 are avirulent to hosts bearing the Gm2 resistance gene (found in rice variety Phalguna), whereas biotype 4 is virulent to Gm2. Based on the sequence of this AFLP marker, SCAR (sequence characterized amplified region) primers were designed and used in combination with previously developed SCAR primers to distinguish effectively all five biotypes in a multiplex PCR-based assay. The inheritance pattern of this marker in the progenies of inter-biotype crosses between biotypes 1, 2 and 4 shows that the marker can be amplified by PCR from all F1 females, irrespective of the biotype status of their parents. However, the marker is present only in those male progenies whose mother was of a Gm2 avirulent biotype. The specific amplification of this marker in the avirulent biotypes and its pattern of inheritance show that avirulence with respect to carriers of the Gm2 gene in rice gall midge is sex-linked.

Key words Sequence-characterized amplified regions (SCARs) Insect resistance DNA fingerprinting Molecular biotyping Rice pest 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • S. K. Behura
    • 1
  • S. Nair
    • 1
  • S. C. Sahu
    • 2
  • M. Mohan
    • 1
  1. 1.International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Road, New Delhi-110 067, India Fax- +91-11-618-7680; E-mail: mohanm@del2.vsnl.net.inIN
  2. 2.Central Rice Research Institute (CRRI), Cuttack-753 006, Orissa, IndiaIN

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