Molecular and General Genetics MGG

, Volume 262, Issue 4, pp 668–676

A new transcriptional activator for amylase genes in Aspergillus

  • K. L. Petersen
  • J. Lehmbeck
  • T. Christensen
ORIGINAL PAPER

DOI: 10.1007/s004380051129

Cite this article as:
Petersen, K., Lehmbeck, J. & Christensen, T. Mol Gen Genet (1999) 262: 668. doi:10.1007/s004380051129

Abstract

We have cloned a regulatory gene for amylase synthesis in Aspergillus oryzae. This gene, amyR, encodes a 604-amino acid transcriptional activator with a Cys6 zinc cluster, that shows extensive homology to the DNA binding domain of GAL4 from Saccharomyces cerevisiae. The DNA binding domain of amyR binds to two types of sequences found in a number of promoters from Aspergillus genes coding for starch-degrading enzymes. One type of binding site is characterized by two CGG triplets separated by eight nucleotides. The other type has only one CGG triplet, which is followed by the sequence AAATTTAA.

Key wordsamyR Amylases Gene regulation Aspergillus oryzae 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • K. L. Petersen
    • 1
  • J. Lehmbeck
    • 1
  • T. Christensen
    • 1
  1. 1.Department of Fungal Genetics, Building 2C, Novo Nordisk, Novo Alle, 2880 Bagsvaerd, Denmark E-mail: toc@novo.dk Fax: +45-44-980246DK

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