Molecular and General Genetics MGG

, Volume 262, Issue 1, pp 121–130

Regulation of divergent transcription from the uvrA-ssb promoters in Sinorhizobium meliloti

  • A. Tapias
  • J. Barbé
ORIGINAL PAPER

DOI: 10.1007/s004380051066

Cite this article as:
Tapias, A. & Barbé, J. Mol Gen Genet (1999) 262: 121. doi:10.1007/s004380051066

Abstract

The Sinorhizobium meliloti uvrA gene was isolated by complementation of a Rhodobacter sphaeroides UvrA mutant. DNA sequencing of the region upstream of the S. meliloti uvrA gene reveals the presence of the ssb gene in the opposite transcriptional orientation. PCR-mediated mutagenesis demonstrated that expression of these two genes is inducible by DNA damage, and depends, in both cases, on the direct repeat GTTCN7GTTC (cited according to the direction of uvrA transcription). Comparison of the sequences of recA and uvrA promoters from different bacterial species of the alpha group of the Proteobacteria has identified the direct repeat GTTCYYKTTTTGTTC as the SOS box in this phylogenetic group.

Key words DNA repairuvrA geneSOS boxα-ProteobacteriaSinorhizobium meliloti

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • A. Tapias
    • 1
  • J. Barbé
    • 1
  1. 1.Molecular Microbiology and Bacterial Genetics Group Department of Genetics and Microbiology Faculty of Sciences, Universitat Autònoma de Barcelona Bellaterra, 08193 Barcelona, Spain e-mail: jbarbe@cc.uab.es Tel.: +34-93-5811837, Fax: +34-93-5812387ES