Molecular and General Genetics MGG

, Volume 261, Issue 6, pp 967–976

Genetic interactions between a null allele of the RIT1 gene encoding an initiator tRNA-specific modification enzyme and genes encoding translation factors in Saccharomyces cerevisiae

Authors

  • S. U. Åström
    • Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden e-mail: Anders.Bystrom@micro.umu.se Tel.:+46-90-7856764; Fax: +46-90-772630
  • M. E. Nordlund
    • Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden e-mail: Anders.Bystrom@micro.umu.se Tel.:+46-90-7856764; Fax: +46-90-772630
  • F. L. Erickson
    • Department of Molecular and Cell Biology, The University of Texas at Dallas, PO Box 830688, Richardson, TX 75083-0688, USA
  • E. M. Hannig
    • Department of Molecular and Cell Biology, The University of Texas at Dallas, PO Box 830688, Richardson, TX 75083-0688, USA
  • A. S. Byström
    • Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden e-mail: Anders.Bystrom@micro.umu.se Tel.:+46-90-7856764; Fax: +46-90-772630
ORIGINAL PAPER

DOI: 10.1007/s004380051045

Cite this article as:
Åström, S., Nordlund, M., Erickson, F. et al. Mol Gen Genet (1999) 261: 967. doi:10.1007/s004380051045

Abstract

The Saccharomyces cerevisiae gene RIT1 encodes a phospho-ribosyl transferase that exclusively modifies the initiator tRNA (tRNAMeti) by the addition of a 2′-O-ribosyl phosphate group to Adenosine 64. As a result, tRNAMeti is prevented from participating in the elongation steps of protein synthesis. We previously showed that the modification is not essential for the function of tRNAMeti in the initiation of translation, since rit1 null strains are viable and show no obvious growth defects. Here, we demonstrate that yeast strains in which a rit1 null allele is combined with mutations in any of the genes for the three subunits of eukaryotic initiation factor-2 (eIF-2), or with disruption alleles of two of the four initiator methionine tRNA (IMT) genes, show synergistic growth defects. A multicopy plasmid carrying an IMT gene can alleviate these defects. On the other hand, introduction of a high-copy-number plasmid carrying the TEF2 gene, which encodes the eukaryotic elongation factor 1α (eEF-1α), into rit1 null strains with two intact IMT genes had the opposite effect, indicating that increased levels of eEF-1α are deleterious to these strains, presumably due to sequestration of the unmodified met-tRNAMeti for elongation. Thus, under conditions in which the components of the ternary met-tRNAMeti:GTP:eIF-2 complex become limiting or are functionally impaired, the presence of the 2′-O-ribosyl phosphate modification in tRNAMeti is important for the provision of adequate amounts of tRNAMeti for formation of this ternary complex.

Key words Initiator tRNA2′-O-Ribosyl phosphate modificationRIT1 geneTranslationSynergistic growth defects

Copyright information

© Springer-Verlag Berlin Heidelberg 1999