Molecular and General Genetics MGG

, Volume 261, Issue 1, pp 184–195

Towards rice genome scanning by map-based AFLP fingerprinting

Authors

  • J. H. Zhu
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • P. Stephenson
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • D. A. Laurie
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • W. Li
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • D. Tang
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • M. D. Gale
    • John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: zhu@bbsrc.ac.uk Tel.: +44-1603-452571; Fax: +44-1603 502241
  • M. T. Jackson
    • International Rice Research Institute, PO Box 933, 1099 Manila, Philippines
ORIGINAL PAPER

DOI: 10.1007/s004380050956

Cite this article as:
Zhu, J., Stephenson, P., Laurie, D. et al. Mol Gen Genet (1999) 261: 184. doi:10.1007/s004380050956

Abstract

Map-based DNA fingerprinting with AFLP markers provides a fast method for scanning the rice genome. Three hundred AFLP markers identified with ten primer combinations were mapped in two rice populations. The genetic maps were aligned and almost full coverage of the rice genome was obtained. The transferability of AFLP markers between indica × japonica and indica × indica crosses was tested. The chromosomes were divided into DNA Fingerprint Linkage Blocks (DFLBs) defined by specific AFLP markers. Using these blocks, the degree of similarity or divergence within specific chromosome regions was calculated for nine varieties. Applications of map-based fingerprinting for biodiversity studies and maker-assisted selection are discussed.

Key words Map-based DNA fingerprintingDNA fingerprint linkage block (DFLB)MappingGenome scanRice

Copyright information

© Springer-Verlag Berlin Heidelberg 1999