Molecular and General Genetics MGG

, Volume 259, Issue 5, pp 549–556

Characterization of the lys2 gene of Penicillium chrysogenum encoding α-aminoadipic acid reductase

  • J. Casqueiro
  • S. Gutiérrez
  • O. Bañuelos
  • F. Fierro
  • J. Velasco
  • J. F. Martín
ORIGINAL PAPER

DOI: 10.1007/s004380050847

Cite this article as:
Casqueiro, J., Gutiérrez, S., Bañuelos, O. et al. Mol Gen Genet (1998) 259: 549. doi:10.1007/s004380050847

Abstract

A DNA fragment containing a gene homologous to LYS2 gene of Saccharomyces cerevisiae was cloned from a genomic DNA library of Penicillium chrysogenum AS-P-78. It encodes a protein of 1409 amino acids (Mr^ 154 859) with strong similarity to the S. cerevisiae (49.9% identity) Schizosaccharomycespombe (51.3% identity) and Candida albicans (48.12% identity) α-aminoadipate reductases and a lesser degree of identity to the amino acid-activating domains of the non-ribosomal peptide synthetases, including the α-aminoadipate-activating domain of the α-aminoadipyl-cysteinyl-valine synthetase of P. chrysogenum (12.4% identical amino acids). The lys2 gene contained one intron in the 5′-region and other in the 3′-region, as shown by comparing the nucleotide sequences of the cDNA and genomic DNA, and was transcribed as a 4.7-kb monocistronic mRNA. The lys2 gene was localized on chromosome III (7.5 Mb) in P. chrysogenum AS-P-78 and on chromosome IV (5.6 Mb) in strain P2, whereas the penicillin gene cluster is known to be located in chromosome I in both strains. The lys2-encoded protein is a member of the aminoacyladenylate-forming enzyme family with a reductase domain in its C-terminal region.

Key wordsα-Aminoadipic acid Lysine biosynthesis Penicillium chrysogenum Reductase domain 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. Casqueiro
    • 1
  • S. Gutiérrez
    • 1
  • O. Bañuelos
    • 1
  • F. Fierro
    • 1
  • J. Velasco
    • 1
  • J. F. Martín
    • 1
  1. 1.Area of Microbiology, Faculty of Biology, University of León 24071 León, and Institute of Biotechnology INBIOTEC Parque Científico de León, Avda. del Real 1, 24006 León, Spain Fax: +34-97-291506; e-mail: degjmm@unileon.esES