Molecular and General Genetics MGG

, Volume 259, Issue 1, pp 105–114

Identification and characterization of the Staphylococcus carnosus nitrate reductase operon

  • I. Pantel
  • P.-E. Lindgren
  • H. Neubauer
  • F. Götz
ORIGINAL PAPER

DOI: 10.1007/s004380050794

Cite this article as:
Pantel, I., Lindgren, P., Neubauer, H. et al. Mol Gen Genet (1998) 259: 105. doi:10.1007/s004380050794

Abstract

Physiological and genetic characterization of Staphylococcus carnosus nitrate reductase-negative mutants led to the identification of the nitrate reductase operon, narGHJI. Transcription from the nar promoter was stimulated by anaerobiosis, nitrate, and nitrite. This is in accordance with the nitrate reductase activities determined with benzyl viologen as electron donor. However, in the presence of oxygen and nitrate, high transcriptional initiation but low nitrate reductase activity was observed. Since the αβ complex of the nitrate reductase formed during anaerobic growth was insensitive to oxygen, other oxygen-sensitive steps (e.g., posttranscriptional mechanisms, molybdenum cofactor biosynthesis) must be involved. The nitrate-reducing system in S. carnosus displays similarities to the dissimilatory nitrate reductases of Escherichia coli. However, in the S. carnosusnar promoter, no obvious Fnr and integration host factor recognition sites are present; only one site that is related to the E. coli NarL consensus sequence was found. Studies to determine whether the E. coli proteins NarL and Fnr are functional at the S. carnosusnarGHJI promoter indicated that the promoter is not functional in E. coli.

Key wordsStaphylococcus carnosusnarGHJI operonNitrate reductaseNarLFnr

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • I. Pantel
    • 1
  • P.-E. Lindgren
    • 1
  • H. Neubauer
    • 1
  • F. Götz
    • 1
  1. 1.Mikrobielle Genetik, Universität Tübingen, Waldhäuserstr. 70/8, D-72076 Tübingen, Germany E-mail: friedrich.goetz@uni-tuebingen.de; Tel.: +49-7071-2974636; Fax: +49-7071-295937DE