Molecular and General Genetics MGG

, Volume 258, Issue 5, pp 553–557

The role of the Aspergillus fumigatusareA gene in invasive pulmonary aspergillosis

Authors

  • M. Hensel
    • Department of Infectious Diseases, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
  • H. N. Arst Jr
    • Department of Infectious Diseases, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
  • A. Aufauvre-Brown
    • Department of Infectious Diseases, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
  • D. W. Holden
    • Department of Infectious Diseases, Imperial College School of Medicine, The Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
ORIGINAL PAPER

DOI: 10.1007/s004380050767

Cite this article as:
Hensel, M., Arst Jr, H., Aufauvre-Brown, A. et al. Mol Gen Genet (1998) 258: 553. doi:10.1007/s004380050767

Abstract

The areA gene of Aspergillus nidulans is a positive-acting transcriptional factor required for the expression of genes involved in the utilization of a broad range of nitrogen sources other than ammonium and glutamine. We have investigated the role in pathogenesis of the corresponding gene (AfareA) of Aspergillus fumigatus, a causative agent of invasive pulmonary aspergillosis. Stable and unstable AfareA strains were constructed and tested for altered virulence in mice on the basis of host survival, mixed infection experiments and genetic reversion studies. These showed that the AfareA gene contributes to, but is not essential for, virulence. Strains carrying extragenic mutations that partially suppress the AfareA phenotype for growth on different nitrogen sources were tested for altered virulence in mixed infection studies. One of these was found to be more virulent than the parental AfareA strain.

Key words invasive pulmonary aspergillosisareAnitrogen utilisationfungal pathogenicity
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© Springer-Verlag Berlin Heidelberg 1998