Molecular and General Genetics MGG

, Volume 258, Issue 3, pp 233–239

A single human cell expresses all messenger ribonucleic acids: the arrow of time in a cell

  • Y. Kimoto
ORIGINAL PAPER

DOI: 10.1007/s004380050727

Cite this article as:
Kimoto, Y. Mol Gen Genet (1998) 258: 233. doi:10.1007/s004380050727

Abstract

Expression of 25 mRNAs in a single human lymphocyte was investigated using the reverse transcription–nested polymerase chain reaction (RT–nested PCR) method. Proteins corresponding to the mRNA investigated were mucin antigen, melanoma antigen, pregnancy-specific β-1 glycoprotein 4, phenylethanolamine-N-methyl-transferase, β B3-crystallin, homeobox 4A, interleukin 2, cluster of differentiation 8, progesterone receptor, parathyroid hormone, gastrin, cholecystokinin/pancreozymin, glucagon, insulin, enkephalin, thyroid stimulating hormone, adrenocorticotropic hormone, synapsin I, immunoglobulin (Ig)M, IgD, IgG1, IgG3, IgE, IgA, and T cell receptor α. All mRNAs were detected in single lymphocytes of two individuals, without exception. In addition, transcripts of IgM, IgD, IgG1, IgG3, IgE, IgA, and the T cell receptor α gene were detected in single sperms. The results strongly suggest the possibility that all mRNAs may be expressed in a single human cell, of both somatic and germ lineage. Thus, cells can consume energy in vain to produce functionally meaningless gene transcripts. However, this basal or illegitimate transcription may be essential for the birth of living matter: the arrow of time in a cell. Moreover, the phenomenon implies the potential of using lymphocytes in place of inaccessible tissue for the diagnosis of genetic diseases.

Key words Illegitimate transcriptionSingle cellLymphocyteSpermArrow of time

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Y. Kimoto
    • 1
  1. 1.Department of Surgical Oncology, Biomedical Research Center, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanJP