ORIGINAL PAPER

Molecular and General Genetics MGG

, Volume 258, Issue 1, pp 1-8

First online:

Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems

  • M. RossiAffiliated withLaboratorio de Fisiología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina Fax: +54-1-780-2788; e-mail: norbius@bg.fcen.uba.ar
  • , F. CarrariAffiliated withLaboratorio de Fisiología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina Fax: +54-1-780-2788; e-mail: norbius@bg.fcen.uba.ar
  • , J. L. Cabrera-PonceAffiliated withCentro de Investigaciones y Estudios Avanzados del I.P.N., Unidad Irapuato, México
  • , C. Vázquez-RovereAffiliated withInstituto de Biotecnología, CICV, Instituto Nacional de Tecnología Agropecuaria, CC 1708 Morón, Argentina
  • , L. Herrera-EstrellaAffiliated withCentro de Investigaciones y Estudios Avanzados del I.P.N., Unidad Irapuato, México
  • , G. GudesblatAffiliated withLaboratorio de Fisiología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina Fax: +54-1-780-2788; e-mail: norbius@bg.fcen.uba.ar
  • , N. D. IusemAffiliated withLaboratorio de Fisiología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina Fax: +54-1-780-2788; e-mail: norbius@bg.fcen.uba.ar

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Abstract

Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.

Key words Tomato Abscisic acid (ABA) Asr genes Asr2 Drought stress