Molecular and General Genetics MGG

, Volume 257, Issue 2, pp 205–212

A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli

Authors

  • M. Dimitrova
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
  • G. Younès-Cauet
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
  • P. Oertel-Buchheit
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
  • D. Porte
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
  • M. Schnarr
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
  • M. Granger-Schnarr
    • Institut de Biologie Moleculaire et Cellulaire, UPR 9002 du CNRS, 15, rue Descartes, F-67084 Strasbourg Cedex, France Fax: +33-3-8860-2218
ORIGINAL PAPER

DOI: 10.1007/s004380050640

Cite this article as:
Dimitrova, M., Younès-Cauet, G., Oertel-Buchheit, P. et al. Mol Gen Genet (1998) 257: 205. doi:10.1007/s004380050640

Abstract

Interactions between proteins affect a wide variety of biological processes, such as signal transduction and control of gene expression. In order to facilitate the study of protein-protein interactions we have developed a new method for specifically detecting the heterodimerization of two heterologous proteins in the bacterium Escherichia coli. The assay is based on the simultaneous use of protein fusions with an altered specificity and a wild-type LexA repressor DNA-binding domain. We have tested this system with two well known eukaryotic dimerization domains (the Fos and Jun leucine zippers). The two interacting proteins were, respectively, fused to a wild-type and a mutant LexA DNA-binding domain. Their hetero-association is specifically measured by the transcriptional repression of a reporter gene (lacZ) controlled by a hybrid operator containing a wild-type half-site (CTGT) and a mutated operator half-site (CCGT). The hybrid operator/lacZ construct was integrated into the chromosome of the reporter strain (SU202) to avoid possible artefacts due to variations in plasmid copy number. This method should be particularly useful in those cases where one or both partners are also able to form homodimers, since the assay described here is sensitive only to the formation of heterodimers. Furthermore, this assay gives rise to a screenable red/white phenotype on MacConkey-lactose indicator plates, allowing for a genetic study of the specificity of the interaction.

Key words LexA repressorFosJunLeucine zipperCoiled coil

Copyright information

© Springer-Verlag Berlin Heidelberg 1998