Molecular and General Genetics MGG

, Volume 256, Issue 6, pp 602–610

Point mutations in the Drosophila sodium channel gene para associated with resistance to DDT and pyrethroid insecticides

  • B. Pittendrigh
  • R. Reenan
  • R. H. ffrench-Constant
  • B. Ganetzky
ORIGINAL PAPER

DOI: 10.1007/s004380050608

Cite this article as:
Pittendrigh, B., Reenan, R., ffrench-Constant, R. et al. Mol Gen Genet (1997) 256: 602. doi:10.1007/s004380050608

Abstract

The gene para in Drosophila melanogaster encodes an α subunit of voltage-activated sodium channels, the presumed site of action of DDT and pyrethroid insecticides. We used an existing collection of Drosophila para mutants to examine the molecular basis of target-site resistance to pyrethroids and DDT. Six out of thirteen mutants tested were associated with a largely dominant, 10- to 30-fold increase in DDT resistance. The amino acid lesions associated with these alleles defined four sites in the sodium channel polypeptide where a mutational change can cause resistance: within the intracellular loop between S4 and S5 in homology domains I and III, within the pore region of homology domain III, and within S6 in homology domain III. Some of these sites are analogous with those defined by knockdown resistance (kdr) and super-kdr resistance-associated mutations in houseflies and other insects, but are located in different homologous units of the channel polypeptide. We find a striking synergism in resistance levels with particular heterozygous combinations of para alleles that appears to mimic the super-kdr double mutant housefly phenotype. Our results indicate that the alleles analyzed from natural populations represent only a subset of mutations that can confer resistance. The implications for the binding site of pyrethroids and mechanisms of target-site insensitivity are discussed.

Key wordspara Voltage-gated sodium channel Insecticide resistance DDT/pyrethroids Drosophila melanogaster 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • B. Pittendrigh
    • 1
  • R. Reenan
    • 2
  • R. H. ffrench-Constant
    • 1
  • B. Ganetzky
    • 2
  1. 1.Department of Entomology and Center for Neuroscience, University of Wisconsin-Madison, 237 Russell Laboratories, 1630 Linden Drive, Madison, WI 53706, USAUS
  2. 2.Department of Genetics, University of Wisconsin-Madison, Henry Mall, Madison, WI 53706, USAUS

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