Molecular and General Genetics MGG

, Volume 256, Issue 5, pp 525–532

The Neisseria gonorrhoeae gene aniA encodes an inducible nitrite reductase

  • J. Mellies
  • J. Jose
  • T. F. Meyer
ORIGINAL PAPER

DOI: 10.1007/s004380050597

Cite this article as:
Mellies, J., Jose, J. & Meyer, T. Mol Gen Genet (1997) 256: 525. doi:10.1007/s004380050597

Abstract

The aniA gene of Neisseria gonorrhoeae encodes an outer membrane lipoprotein which is strongly induced when gonococci are grown anaerobically in vitro in the presence of nitrite. Database searches with the amino acid sequence derived from the aniA structural gene revealed significant homologies to copper-containing nitrite reductases from several denitrifying bacteria. We constructed an insertional mutation in the aniA locus of strain MS11 by allelic replacement, to determine whether this locus was necessary for growth in oxygen-depleted environments, and to demonstrate that AniA was indeed a nitrite reductase. The mutant was severely impaired in its ability to grow microaerophilically in the presence of nitrite, and we observed a loss in viability over several hours of incubation. No measurable nitrite reductase activity was detected in the aniA mutant strain, and activity in the strain with a wild-type locus was inducible. Finally, we report investigations to determine whether AniA protein is involved in gonococcal pathogenesis.

Key words GonococcianiAPan1Nitrite reductaseAnaerobiosis

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • J. Mellies
    • 1
  • J. Jose
    • 1
  • T. F. Meyer
    • 1
  1. 1.Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Spemannstrasse 34, D-72076 Tübingen, Germany Fax: +49-7071-610-379DE
  2. 2.Max-Planck-Institut für Infektionsbiologie, Abteilung Molekulare Biologie, Monbijoustrasse 2, D-10117 Berlin, GermanyDE