Molecular and General Genetics MGG

, Volume 256, Issue 3, pp 231–238

Genetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis : combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules

  • H. Tsukaya
  • H. Uchimiya

DOI: 10.1007/s004380050565

Cite this article as:
Tsukaya, H. & Uchimiya, H. Mol Gen Genet (1997) 256: 231. doi:10.1007/s004380050565


Developmental control of the formation of the serrated margin of leaf blades was investigated. First, the expression was characterized of a marker gene encoding β-glucuronidase in strain #1-35-38, a transgenic strain of Arabidopsis thaliana (L.) Heynh, derived by the use of a previously described transposon-tagging system. In strain #1-35-38, expression of the marker gene was tissue-specific, being restricted to stipules and the toothed margins of laminae. Using this transgenic marker gene, we examined the development of leaf blade margins in Arabidopsis. We compared the pattern of expression of the marker gene in the leaves of the wild-type plant with that in plants carrying the asymmetric leaves1 (as1) mutation, which causes dramatic changes in leaf-blade morphology in Arabidopsis. The as1 mutant showed normal morphology of early leaf primordia. The mutation affected the development of leaf segmentation in Arabidopsis without any change in the number or morphology of cells in laminae. The as1 mutation affected leaf morphology independently of mutations in other genes known to affect leaf morphogenesis, such as the acaulis1 mutation and the angustifolia mutation. Based upon these results, the development of the morphology of leaf margins in Arabidopsis is discussed.

Key wordsas1 mutantGUS marker geneHydathodeLeaf morphogenesisLeaf serration

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • H. Tsukaya
    • 1
  • H. Uchimiya
    • 1
  1. 1.Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan Fax: +81-3-3812-2910 e-mail: