ORIGINAL PAPER

Molecular and General Genetics MGG

, Volume 255, Issue 4, pp 438-447

First online:

Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes

  • J. N. A. M. Rouppe van der VoortAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
  • , P. van ZandvoortAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
  • , H. J. van EckAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University Department of Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands
  • , R. T. FolkertsmaAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
  • , R. C. B. HuttenAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University Department of Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands
  • , J. DraaistraAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
  • , F. J. GommersAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
  • , E. JacobsenAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University Department of Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands
  • , J. HelderAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands
    • , J. BakkerAffiliated withThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands

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Abstract

The allele specificity of AFLP markers was assessed in five relatively unrelated potato genotypes. To this end, two diploid mapping populations of potato, F1SH × RH and F1AM × RH, were analysed using four and six AFLP primer combinations, respectively, recently applied to the analysis of the genetically well characterized backcross population BC_C × E. The AFLP profiles of the five parents revealed 733 AFLP markers and, when identical primer combinations were used, 131 comigrating AFLP markers were identified. After construction of five parental maps, the genomic positions of these comigrating AFLP markers were compared and 117 markers (89%) which targeted the same genomic region were assumed to be homologous. Of these putative homologues, 20 markers, each cloned from at least two genotypes, were sequenced and 19 sets of amplification products were shown to be nearly identical. The number of AFLP markers previously mapped in population BC_C × E ranged from three to eleven per chromosome, which allowed a reliable assessment of chromosome numbers from individual linkage groups obtained in populations F1SH × RH and F1AM × RH. The high incidence of corresponding AFLP alleles was confirmed by using an additional set of five primer combinations. The 733 AFLP markers localized provide a valuable reference collection for future mapping studies in potato. As a consequence AFLP analysis may replace more laborious locus-specific marker techniques.

Key words Comparative mapping DNA markers Solanum tuberosum