Molecular and General Genetics MGG

, Volume 254, Issue 5, pp 571–577

The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins

  • F. Sladeczek
  • J. H. Camonis
  • A.-F. Burnol
  • F. Le Bouffant
ORIGINAL PAPER

DOI: 10.1007/s004380050453

Cite this article as:
Sladeczek, F., Camonis, J., Burnol, AF. et al. Mol Gen Genet (1997) 254: 571. doi:10.1007/s004380050453

Abstract

PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 (also known as calpactin I light chain) and the η, θ (also known asτ) and ζ isoforms of 14-3-3 proteins. We confirmed that these four proteins interact with PCTAIRE-1 by demonstrating the biochemical interactions using the pure recombinant proteins. The fact that 14-3-3 proteins are known to interact with many other intracellular proteins (such as C-kinase, Raf, Bcr, PI3-kinase) and p11 with annexin II (a major pp60v-src and C-kinase substrate) suggests that PCTAIRE-1 might be part of multiple signal transduction cascades and cellular protein networks.

Key words Cdk-like protein PCTAIRE-1   Two-hybrid system  Protein-protein interactions   Annexin II-associated protein p11   14-3-3 proteins

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • F. Sladeczek
    • 1
  • J. H. Camonis
    • 2
  • A.-F. Burnol
    • 3
  • F. Le Bouffant
    • 1
  1. 1.CNRS UPR 2212, Institut Alfred Fessard, Avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex
  2. 2.INSERM U-248, Section de Recherche, Institut Curie, 26 rue d'Ulm, F-75231 Paris Cedex 05
  3. 3.Centre de Recherches en Endocrinologie Moléculaire et Développement, CNRS, 9 rue Jules Hetzel, F-92190 Meudon-Bellevue