Molecular and General Genetics MGG

, Volume 254, Issue 3, pp 275–283

Cycloheximide induces a subset of low temperature-inducible genes in maize

  • T. Berberich
  • T. Kusano
ORIGINAL PAPER

DOI: 10.1007/s004380050416

Cite this article as:
Berberich, T. & Kusano, T. Mol Gen Genet (1997) 254: 275. doi:10.1007/s004380050416

Abstract

To investigate further the signal transduction pathway that mediates the cold-stress response in maize, we isolated a low temperature-inducible cDNA clone (ZmCDPK1) that encodes a calcium-dependent protein kinase. Time-course experiments revealed that the low-temperature induction of ZmCDPK1 precedes that of mlip15, another cold-inducible gene that codes for a DNA-binding protein of the basic region/leucine zipper (bZIP) type, indicating that ZmCDPK1 might be located upstream of mlip15 in the cold-stress signal transduction pathway. We observed that the steady-state mRNA level of mlip15 drastically increased after cycloheximide treatment. In addition to mlip15, cycloheximide elevates the transcript levels of two other low temperature-induced genes, ZmCDPK1, and Adh1, which encodes alcohol dehydrogenase 1. In contrast, the chalcone synthase gene was only inducible by low temperature. The accumulation of the mlip15 transcript at low temperatures and in response to cycloheximide was significantly reduced by pretreatment with a calcium chelator, suggesting that calcium is involved in both cases of mlip15 induction. The signal transduction pathways triggered by low temperature and cycloheximide are discussed in relation to these observations.

Key words Low temperature  Cycloheximide  Maize Calcium  Calcium-dependent protein kinase

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • T. Berberich
    • 1
  • T. Kusano
    • 1
  1. 1.Biotechnology Institute, Akita Prefectural College of Agriculture, Ohgata, Akita 010-04, JapanJP