Molecular and General Genetics MGG

, Volume 253, Issue 4, pp 520–528

The Aspergillus nidulans genes chsA and chsD encode chitin synthases which have redundant functions in conidia formation

Authors

  • T. Motoyama
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
  • Makoto Fujiwara
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
  • Nobuko Kojima
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
  • Hiroyuki Horiuchi
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
  • Akinori Ohta
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
  • M. Takagi
    • Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
ORIGINAL PAPER/CORRIGENDUM

DOI: 10.1007/s004380050353

Cite this article as:
Motoyama, T., Fujiwara, M., Kojima, N. et al. Mol Gen Genet (1997) 253: 520. doi:10.1007/s004380050353

Abstract

 We previously isolated three chitin synthase genes (chsA, chsB, and chsC) from Aspergillus nidulans. In the present work, we describe the isolation and characterization of another chitin synthase gene, named chsD, from A. nidulans. Its deduced amino acid sequence shows 56.7% and 55.9% amino acid identity, respectively, with Cal1 of Saccharomyces cerevisiae and Chs3 of Candida albicans. Disruption of chsD caused no defect in cell growth or morphology during the asexual cycle and caused no decrease in chitin content in hyphae. However, double disruption of chsA and chsD caused a remarkable decrease in the efficiency of conidia formation, while double disruption of chsC and chsD caused no defect. Thus it appears that chsA and chsD serve redundant functions in conidia formation.

Key words Chitin synthaseMultigene familyCell wallConidiaAspergillus nidulans
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© Springer-Verlag Berlin Heidelberg 1997