Original Paper

Molecular Genetics and Genomics

, Volume 265, Issue 1, pp 126-134

First online:

Hsp90 levels affect telomere length in yeast

  • N. GrandinAffiliated withYeast Cell Cycle Group, UMR CNRS 5665, Ecole Normale Supérieure, 46, Allée d'Italie, 69364 Lyon
  • , M. CharbonneauAffiliated withYeast Cell Cycle Group, UMR CNRS 5665, Ecole Normale Supérieure, 46, Allée d'Italie, 69364 Lyon

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Abstract

Cdc13 is a Saccharomyces cerevisiae protein that binds to telomeric single-stranded DNA and regulates telomerase activity. Stn1 has been shown by two-hybrid analysis to form a physical complex with Cdc13. Temperature-sensitive mutations in CDC13 and STN1, which are both essential genes, activate a DNA damage-dependent checkpoint which is the cause of the arrest seen in the mutant strains. The stn1-13 mutation induces dramatic telomere elongation which is telomerase dependent, as shown here. Additional mutants for STN1, which show a tighter arrest phenotype than stn1-13, were generated in order to perform genetic screens aiming at uncovering new regulators of telomerase. HSC82, which encodes a conserved molecular chaperone of the Hsp90 family, was thus isolated as a high-dosage suppressor of a temperature-sensitive mutation in STN1. Overexpression of HSC82 also partially suppressed the growth defect of cdc13-1 cells. Overexpression of HSC82 was found to correct the telomeric defect associated with stn1 mutations. Shortening of telomeres was also observed in wild-type cells upon overexpression of HSC82, or of its temperature-inducible homologue, HSP82. These results identify Hsc82/Hsp82 as potential regulators of telomerase in yeast cells.

Telomere length Hsc82 Hsp82 STN1 CDC13