Molecular and General Genetics MGG

, Volume 264, Issue 3, pp 317–324

Post-translational regulation of Saccharomyces cerevisiae proteins tagged with the hormone-binding domains of mammalian nuclear receptors

Authors

  • H. Launhardt
    • Department of Cell and Molecular Biology, Hans-Knöll-Institut für Naturstoff-Forschung e.V., Beutenbergstr. 11, D-07745 Jena, Germany
  • T. Munder
    • Department of Cell and Molecular Biology, Hans-Knöll-Institut für Naturstoff-Forschung e.V., Beutenbergstr. 11, D-07745 Jena, Germany
Original Paper

DOI: 10.1007/s004380000325

Cite this article as:
Launhardt, H. & Munder, T. Mol Gen Genet (2000) 264: 317. doi:10.1007/s004380000325

Abstract.

In the post-genome sequencing era the functional analysis of newly discovered proteins becomes more and more important. In this report we describe a genetic approach to the post-translational regulation of protein function in Saccharomyces cerevisiae by creating conditional lethal mutants. The yeast ORFs YDL139c, YDL147w, ERG3 and ERG11 were tagged with sequences encoding the hormone-binding domains of mammalian steroid receptors by PCR-mediated, targeted integration into the yeast genome. We found that the function of the chimeric proteins is regulated in a hormone-dependent way. This technique provides another important tool for the functional analysis of the yeast proteome.

Essential genes Hormone-binding domain Mammalian nuclear receptors PCR-based integration Saccharomyces cerevisiae

Copyright information

© Springer-Verlag 2000