Molecular and General Genetics MGG

, Volume 264, Issue 3, pp 283–290

Physical mapping of the barley stem rust resistance gene rpg4

  • A. Druka
  • D. Kudrna
  • F. Han
  • A. Kilian
  • B. Steffenson
  • D. Frisch
  • J. Tomkins
  • R. Wing
  • A. Kleinhofs
Original Paper

DOI: 10.1007/s004380000320

Cite this article as:
Druka, A., Kudrna, D., Han, F. et al. Mol Gen Genet (2000) 264: 283. doi:10.1007/s004380000320

Abstract.

The barley stem rust resistance gene rpg4 was physically and genetically localized on two overlapping BAC clones covering an estimated 300-kb region of the long arm of barley chromosome 7(5H). Initially, our target was mapped within a 6.0-cM region between the previously described flanking markers MWG740 and ABG391. This region was then saturated by integrating new markers from several existing barley and rice maps and by using BAC libraries of barley cv. Morex and rice cv. Nipponbare. Physical/genetic distances in the vicinity of rpg4 were found to be 1.0 Mb/cM, which is lower than the average for barley (4 Mb/cM) and lower than that determined by translocation breakpoint mapping (1.8 Mb/cM). Synteny at high resolution levels has been established between the region of barley chromosome 7(5H) containing the rpg4 locus and the subtelomeric region of rice chromosome 3 between markers S16474 and E10757. This 1.7-cM segment of the rice genome was covered by two overlapping BAC clones, about 250 kb of total length. In barley the markers S16474 and E10757 genetically delimit rpg4, lying 0.6 cM distal and 0.4 cM proximal to the locus, respectively.

Map-based cloning Resistance genes Barley genome Stem rust

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • A. Druka
    • 1
  • D. Kudrna
    • 1
  • F. Han
    • 1
  • A. Kilian
    • 2
  • B. Steffenson
    • 3
  • D. Frisch
    • 4
  • J. Tomkins
    • 4
  • R. Wing
    • 4
  • A. Kleinhofs
    • 1
  1. 1.Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA
  2. 2.Center for the Application of Molecular Biology to International Agriculture, GPO Box 3200, Canberra ACT 2601, Australia
  3. 3.Department of Plant Pathology, North Dakota State University, P.O. Box 5012, Fargo, ND 581055012, USA
  4. 4.Genomics Institute, Clemson University, 100 Jordan Hall, Clemson, SC 29634, USA
  5. 5.School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
  6. 6.Present address: Pioneer Hi-Bred International, Inc., 7300 NW 62nd Ave., Johnston, IA 50131 USA