Molecular and General Genetics MGG

, Volume 263, Issue 6, pp 898-907

First online:

Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in Pisum

  • S. R. PearceAffiliated withPlant Stress Unit, School of Biology, University of Sussex, Falmer, Brighton BN1 9QG, UK e-mail: S.R.Pearce@sussex.ac.uk Tel.: +44-1273-678872; Fax: +44-1273-678433
  • , M. KnoxAffiliated withDepartment of Applied Genetics, John Innes Centre, Norwich NR4 7UH, UK
  • , T. H. N. EllisAffiliated withDepartment of Applied Genetics, John Innes Centre, Norwich NR4 7UH, UK
  • , A. J. FlavellAffiliated withDepartment of Biochemistry, MSI/WTB, University of Dundee, Dundee DD1 5EH, UK
  • , A. KumarAffiliated withScottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The variation in transposition history of different Ty1-copia group LTR retrotransposons in the species lineages of the Pisum genus has been investigated. A heterogeneous population of Ty1-copia elements was isolated by degenerate PCR and two of these (Tps12 and Tps19) were selected on the basis of their copy number and sequence conservation between closely related species for further in-depth study of their transpositional history in Pisum species. The insertional polymorphism of these elements and the previously characterised PDR1 element was studied by sequence-specific amplification polymorphism (SSAP). Each of these elements reveals a unique transpositional history within 55 diverse Pisum accessions. Phylogenetic trees based on the SSAP data show that SSAP markers for individual elements are able to resolve different species lineages within the Pisum genus. Finally, the SSAP data from all of these retrotransposon markers were combined to reveal a detailed picture of the intra and inter-species relationships within Pisum.

Key words Retrotransposon Genetic diversity Molecular taxonomy Pea DNA markers