Molecular Genetics and Genomics

, Volume 283, Issue 3, pp 289–303

AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans

Authors

  • Anita Balázs
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
  • Imre Pócsi
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
    • Department of Microbiology, Faculty of Science and InformaticsUniversity of Szeged
  • Éva Leiter
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
  • Tamás Emri
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
  • Márton Miskei
    • Department of Horticulture and Plant Biotechnology, Faculty of Agricultural ScienceUniversity of Debrecen
  • Judit Oláh
    • Department of Horticulture and Plant Biotechnology, Faculty of Agricultural ScienceUniversity of Debrecen
  • Viktória Tóth
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
  • Nikoletta Hegedűs
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
  • Rolf A. Prade
    • Department of Microbiology and Molecular GeneticsOklahoma State University
  • Mónika Molnár
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
    • Department of Microbial Biotechnology and Cell Biology, Faculty of Science and TechnologyUniversity of Debrecen
Original Paper

DOI: 10.1007/s00438-010-0513-z

Cite this article as:
Balázs, A., Pócsi, I., Hamari, Z. et al. Mol Genet Genomics (2010) 283: 289. doi:10.1007/s00438-010-0513-z

Abstract

The aim of the study was to demonstrate that the bZIP-type transcription factor AtfA regulates different types of stress responses in Aspergillus nidulans similarly to Atf1, the orthologous ‘all-purpose’ transcription factor of Schizosaccharomyces pombe. Heterologous expression of atfA in a S. pombe Δatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation of the osmotic and oxidative-stress-sensitive phenotypes was also achieved in submerged cultures. AtfA is therefore a true functional ortholog of fission yeast’s Atf1. As demonstrated by RT-PCR experiments, elements of both oxidative (e.g. catalase B) and osmotic (e.g. glycerol-3-phosphate dehydrogenase B) stress defense systems were transcriptionally regulated by AtfA in a stress-type-specific manner. Deletion of atfA resulted in oxidative-stress-sensitive phenotypes while the high-osmolarity stress sensitivity of the fungus was not affected significantly. In A. nidulans, the glutathione/glutathione disulfide redox status of the cells as well as apoptotic cell death and autolysis seemed to be controlled by regulatory elements other than AtfA. In conclusion, the orchestrations of stress responses in the aspergilli and in fission yeast share several common features, but further studies are needed to answer the important question of whether a fission yeast-like core environmental stress response also operates in the euascomycete genus Aspergillus.

Keywords

Stress signalingOxidative stressOsmotic stressApoptosisAutolysisAuxotrophyCESR

Copyright information

© Springer-Verlag 2010