Molecular Genetics and Genomics

, Volume 280, Issue 4, pp 337–350

Functional features of the C-terminal region of yeast ribosomal protein L5

  • Hossein Moradi
  • Ivailo Simoff
  • Galyna Bartish
  • Odd Nygård
Original Paper

DOI: 10.1007/s00438-008-0369-7

Cite this article as:
Moradi, H., Simoff, I., Bartish, G. et al. Mol Genet Genomics (2008) 280: 337. doi:10.1007/s00438-008-0369-7

Abstract

The aim of this study was to analyze the functional importance of the C-terminus of the essential yeast ribosomal protein L5 (YrpL5). Previous studies have indicated that the C-terminal region of YrpL5 forms an α-helix with a positively charged surface that is involved in protein–5S rRNA interaction. Formation of an YrpL5·5S rRNA complex is a prerequisite for nuclear import of YrpL5. Here we have tested the importance of the α-helix and the positively charged surface for YrpL5 function in Saccharomyces cerevisiae using site directed mutagenesis in combination with functional complementation. Alterations in the sequence forming the putative α-helix affected the functional capacity of YrpL5. However, the effect did not correlate with a decreased ability of the protein to bind to 5S rRNA as all rpL5 mutants tested were imported to the nucleus whether or not the α-helix or the positively charged surface were intact. The alterations introduced in the C-terminal sequence affected the growth rate of cells expressing mutant but functional forms of YrpL5. The reduced growth rate was correlated with a reduced ribosomal content per cell indicating that the alterations introduced in the C-terminus interfered with ribosome assembly.

Keywords

Functional complementationMutation analysisRibosomal protein L5S. cerevisiae

Abbreviations

YrpL5

Yeast ribosomal protein L5

Gal

Galactose

Glu

Glucose

SC

Synthetic complete

5FOA

5-Fluorortic acid

RNP

Ribonucleoprotein

SDS

Sodium dodecyl sulphate

Supplementary material

438_2008_369_MOESM1_ESM.pdf (152 kb)
MOESM1 [INSERT CAPTION HERE] (PDF 151 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Hossein Moradi
    • 1
    • 2
  • Ivailo Simoff
    • 1
    • 2
  • Galyna Bartish
    • 1
    • 2
  • Odd Nygård
    • 2
  1. 1.Department of Cell Biology, Arrhenius Laboratories E5Stockholm UniversityStockholmSweden
  2. 2.Cell Biology Unit, Department of Life SciencesSödertörns högskolaHuddingeSweden