Original Paper

Molecular Genetics and Genomics

, Volume 279, Issue 3, pp 279-289

First online:

Identification, characterization and interaction of HAP family genes in rice

  • Thiruvengadam ThirumuruganAffiliated withGenetic Strains Research Center, National Institute of GeneticsSchool of Life Science, Graduate University for Advanced Studies
  • , Yukihiro ItoAffiliated withGenetic Strains Research Center, National Institute of GeneticsGraduate School of Agricultural Science, Tohoku University
  • , Takahiko KuboAffiliated withGenetic Strains Research Center, National Institute of Genetics
  • , Akiko SerizawaAffiliated withGenetic Strains Research Center, National Institute of Genetics
  • , Nori KurataAffiliated withGenetic Strains Research Center, National Institute of GeneticsSchool of Life Science, Graduate University for Advanced Studies Email author 

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Abstract

A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

Keywords

CCAAT-box HAP2/NF-YA/CBF-B HAP3/NF-YB/CBF-A HAP5/NF-YC/CBF-C Protein–protein interaction Rice