Molecular Genetics and Genomics

, Volume 279, Issue 2, pp 123–132

Different physiological relevance of yeast THO/TREX subunits in gene expression and genome integrity

  • María García-Rubio
  • Sebastián Chávez
  • Pablo Huertas
  • Cristina Tous
  • Sonia Jimeno
  • Rosa Luna
  • Andrés Aguilera
Original Paper

DOI: 10.1007/s00438-007-0301-6

Cite this article as:
García-Rubio, M., Chávez, S., Huertas, P. et al. Mol Genet Genomics (2008) 279: 123. doi:10.1007/s00438-007-0301-6

Abstract

THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and plays a role in preventing the transcription-associated genetic instability. THO is composed of Tho2, Hpr1, Mft1 and Thp2 subunits, which associate with the Sub2-Yra1 export factors and Tex1 to form the TREX complex. To compare the functional relevance of the different THO/TREX subunits, we determined the effect of their null mutations on mRNA accumulation and recombination. Unexpectedly, we noticed that a full deletion of HPR1, hpr1ΔK, conferred stronger hyper-recombination phenotype and gene expression defects than did hpr1ΔH, the allele encoding a C-terminal truncated protein which was used in most previous studies. We show that tho2Δ and, to a lesser extent, hpr1ΔK are the THO mutations with the highest impact on all phenotypes, and that sub2Δ shows a similar transcription-dependent hyper-recombination phenotype and in vivo transcription impairment as hpr1ΔK and tho2Δ. Recombination and transcription analyses indicate that THO/TREX mutants share a moderate but significant effect on gene conversion and ectopic recombination, as well as transcription impairment of even short and low GC-content genes. Our data provide new information on the relevance of these proteins in mRNP biogenesis and in the maintenance of genomic integrity.

Keywords

THO complexSub2Genetic instabilitymRNP biogenesisTranscriptionTranscription-associated recombination

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • María García-Rubio
    • 1
    • 2
  • Sebastián Chávez
    • 2
  • Pablo Huertas
    • 2
    • 3
  • Cristina Tous
    • 1
    • 2
  • Sonia Jimeno
    • 1
    • 2
  • Rosa Luna
    • 1
    • 2
  • Andrés Aguilera
    • 1
    • 2
  1. 1.Departamento de Biología Molecular, CABIMERCSIC, Universidad de SevillaSevillaSpain
  2. 2.Departamento de Genética, Facultad de BiologíaUniversidad de SevillaSevillaSpain
  3. 3.The Wellcome Trust and Cancer Research UK Gurdon InstituteUniversity of CambridgeCambridgeUK