Molecular Genetics and Genomics

, Volume 279, Issue 1, pp 1–10

Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize

  • André Beló
  • Peizhong Zheng
  • Stanley Luck
  • Bo Shen
  • David J. Meyer
  • Bailin Li
  • Scott Tingey
  • Antoni Rafalski
Original Paper

DOI: 10.1007/s00438-007-0289-y

Cite this article as:
Beló, A., Zheng, P., Luck, S. et al. Mol Genet Genomics (2008) 279: 1. doi:10.1007/s00438-007-0289-y

Abstract

We used whole genome scan association mapping to identify loci with major effect on oleic acid content in maize kernels. Single nucleotide polymorphism haplotypes at 8,590 loci were tested for association with oleic acid content in 553 maize inbreds. A single locus with major effect on oleic acid was mapped between 380 and 384 cM in the IBM2 neighbors genetic map on chromosome 4 and confirmed in a biparental population. A fatty acid desaturase, fad2, identified ∼2 kb from the associated genetic marker, is the most likely candidate gene responsible for the differences in the phenotype. The fad2 alleles with high- and low-oleic acid content were sequenced and allelic differences in fad2 RNA level in developing embryos was investigated. We propose that a non-conservative amino acid polymorphism near the active site of fad2 contributes to the effect on oleic acid content. This is the first report of the use of a high resolution whole genome scan association mapping where a putative gene responsible for a quantitative trait was identified in plants.

Keywords

Whole genome scan Association mapping Maize fad2 Oleic fatty acid 

Supplementary material

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • André Beló
    • 1
    • 3
  • Peizhong Zheng
    • 2
  • Stanley Luck
    • 3
  • Bo Shen
    • 2
  • David J. Meyer
    • 2
  • Bailin Li
    • 3
  • Scott Tingey
    • 3
  • Antoni Rafalski
    • 1
    • 3
  1. 1.Graduate Integrative Training Option program, Department of Plant and Soil SciencesUniversity of DelawareNewarkUSA
  2. 2.Pioneer Hi-Bred InternationalJohnstonUSA
  3. 3.DuPont Crop Genetics ResearchDuPont Experimental Station E353WilmingtonUSA

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