Molecular Genetics and Genomics

, Volume 276, Issue 2, pp 162–169

AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize

Authors

  • Z. F. Zhang
    • National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University
  • Y. Wang
    • National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University
    • National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural University
Original Paper

DOI: 10.1007/s00438-006-0131-y

Cite this article as:
Zhang, Z.F., Wang, Y. & Zheng, Y.L. Mol Genet Genomics (2006) 276: 162. doi:10.1007/s00438-006-0131-y

Abstract

The Rf3 gene restores the pollen fertility disturbed by S male sterile cytoplasm. In order to develop molecular markers tightly linked to Rf3, we used amplified fragment length polymorphism (AFLP) technique with near isogenic lines (NILs) and bulk segregant analysis (BSA). A BC1F1 population from a pair of NILs with different Rf3 locus was constructed and 528 primer combinations was screened. A linkage map was constructed around the Rf3 locus, which was mapped on the distal region of chromosome 2 long arm with the help of SSR marker UMC2184. The closest marker E7P6 was 0.9 cM away from Rf3. Marker E3P1, 2.4 cM from Rf3, and E12M7, 1.8 cM from Rf3, were converted into a codominant CAPS and a dominant SCAR marker, and designated as CAPSE3P1 and SCARE12M7, respectively. These markers are useful for marker-assisted selection and map-based cloning of the Rf3 gene.

Keywords

MaizeFertility restorerNear isogenic linesBulk segregant analysisAFLPPCR-based markersMarker-assisted selectionMap-based cloning

Abbreviations

AFLP

Amplified fragment length polymorphism

BF

Fertile DNA bulks

BS

Sterile DNA bulks

BSA

Bulk segregant analysis

CAPS

Cleaved amplified polymorphic sequence

CMS

Cytoplasmic male sterility

E/M

EcoRI/MseI

P/M

PstI/MseI

P/E

EcoRI/PstI

MAS

Marker-assisted selection

NILs

Near isogenic lines

ORF

Open reading frames

PC

Primer combinations

PPR

Pentatricopeptide repeat

RAPD

Random amplified polymorphic DNA

RFLP

Restriction fragment length polymorphism

SCAR

Sequence characterized amplified regions

SNP

Singe nucleotide polymorphism

SSR

Simple sequence repeat, microsatellite

Copyright information

© Springer-Verlag 2006