Molecular Genetics and Genomics

, Volume 273, Issue 3, pp 273–281

A genetic linkage map of the soybean cyst nematode Heterodera glycines

  • N. Atibalentja
  • S. Bekal
  • L. L. Domier
  • T. L. Niblack
  • G. R. Noel
  • K. N. Lambert
Original Paper

DOI: 10.1007/s00438-005-1125-x

Cite this article as:
Atibalentja, N., Bekal, S., Domier, L.L. et al. Mol Genet Genomics (2005) 273: 273. doi:10.1007/s00438-005-1125-x

Abstract

A genetic linkage map of the soybean cyst nematode (SCN) Heterodera glycines was constructed using a population of F2 individuals obtained from matings between two highly inbred SCN lines, TN16 and TN20. The AFLP fingerprinting technique was used to genotype 63 F2 progeny with two restriction enzyme combinations (EcoRI/MseI and PstI/TaqI) and 38 primer combinations. The same F2 population was also genotyped for Hg-cm-1 (H. glycines chorismate mutase-1), a putative virulence gene, using real-time quantitative PCR. Some of the markers were found to be distributed non-randomly. Even so, of the 230 markers analyzed, 131 could be mapped onto ten linkage groups at a minimum LOD of 3.0, for a total map distance of 539 cM. The Hg-cm-1 locus mapped to linkage group III together with 16 other markers. The size of the H. glycines genome was estimated to be in the range of 630–743 cM, indicating that the current map represents 73–86% of the genome, with a marker density of one per 4.5 cM, and a physical/genetic distance ratio of between 124 kb/cM and 147 kb/cM. This genetic map will be of great assistance in mapping H. glycines markers to genes of interest, such as nematode virulence genes and genes that control aspects of nematode parasitism.

Keywords

AFLP markers Real-time PCR Plant pathogen Whole-genome amplification 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • N. Atibalentja
    • 1
  • S. Bekal
    • 1
  • L. L. Domier
    • 1
    • 2
  • T. L. Niblack
    • 1
  • G. R. Noel
    • 1
    • 2
  • K. N. Lambert
    • 1
  1. 1.Department of Crop SciencesUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.USDAARSUrbanaUSA

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