Molecular Genetics and Genomics

, Volume 273, Issue 2, pp 158–166

Isopentenyl-diphosphate isomerase is essential for viability of Caenorhabditis elegans

  • John Yochem
  • David H. Hall
  • Leslie R. Bell
  • Edward M. Hedgecock
  • Robert K. Herman
Original Paper

DOI: 10.1007/s00438-004-1101-x

Cite this article as:
Yochem, J., Hall, D.H., Bell, L.R. et al. Mol Genet Genomics (2005) 273: 158. doi:10.1007/s00438-004-1101-x

Abstract

Homozygosity for a mutation in the idi-1 gene of Caenorhabditis elegans results in paralysis during the first larval stage, followed by an arrest of growth and development late in the first larval stage. Apoptotic corpses, which are apparently the result of normal programmed cell death, persist in the arrested larvae. In genetic mosaics, an additional defect becomes evident upon examination with Nomarski optics: cells that are genotypically mutant enlarge, and their cytoplasm becomes dimpled. Electron microscopy indicates that the dimpling reflects an accumulation of many enlarged lysosomes and autophagosomes. The mosaics demonstrate that the lethal mutation acts cell autonomously with respect to this vesicular abnormality and that there is a maternal effect with respect to the time of developmental arrest of mutant progeny. Cloning of the gene reveals that it is the only gene in C. elegans for isopentenyl-diphosphate isomerase, an enzyme that is important for the synthesis of lipophilic molecules, including farnesyl and geranyl diphosphates.

Keywords

idi-1PrenylationAutophagyLysosomal storage disease

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • John Yochem
    • 1
  • David H. Hall
    • 2
  • Leslie R. Bell
    • 1
  • Edward M. Hedgecock
    • 3
  • Robert K. Herman
    • 1
  1. 1.Department of Genetics, Cell Biology and DevelopmentUniversity of MinnesotaMinneapolisUSA
  2. 2.Center for C. elegans AnatomyAlbert Einstein College of MedicineBronxUSA
  3. 3.Department of BiologyJohns Hopkins UniversityBaltimoreUSA