Molecular Genetics and Genomics

, Volume 272, Issue 6, pp 639–650

A H2O2-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis

  • B. Leuthner
  • C. Aichinger
  • E. Oehmen
  • E. Koopmann
  • O. Müller
  • P. Müller
  • R. Kahmann
  • M. Bölker
  • P. H. Schreier
Original Paper

DOI: 10.1007/s00438-004-1085-6

Cite this article as:
Leuthner, B., Aichinger, C., Oehmen, E. et al. Mol Genet Genomics (2005) 272: 639. doi:10.1007/s00438-004-1085-6

Abstract

In the phytopathogenic fungus Ustilago maydis the mating-type loci control the transition from yeast-like to filamentous growth required for pathogenic development. In a large REMI (restriction enzyme mediated integration) screen, non-pathogenic mutants were isolated in a haploid strain that had been engineered to be pathogenic. In one of these mutants, which showed a specific morphological phenotype, the tagged gene, glo1 , was found to encode a product that is highly homologous to a glyoxal oxidase gene from the wood-rot fungus Phanerochaete chrysosporium. Glyoxal oxidase homologues are found in human, plant pathogenic fungi and in plants, but not in other mammals or yeasts. To confirm the function of the glo1 gene, null mutations were generated in compatible haploid U. maydis strains. In crosses null mutants were unable to generate filamentous dikaryons, and were completely non-pathogenic. Using a Glo1-overproducing strain we demonstrated that Glo1 is membrane bound, oxidizes a series of small aldehydes (<C4) and produces H2O2. The enzyme needs to be activated, presumably by auto-oxidation, to show full activity. A potential role for Glo1 during filamentous growth and pathogenic development of U. maydis is proposed.

Keywords

Deletion mutant Gene knock-out Overproduction Protein 

Supplementary material

supp.pdf (318 kb)
(PDF 318 KB)

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • B. Leuthner
    • 1
    • 5
  • C. Aichinger
    • 1
    • 6
  • E. Oehmen
    • 1
  • E. Koopmann
    • 1
  • O. Müller
    • 2
  • P. Müller
    • 3
  • R. Kahmann
    • 3
  • M. Bölker
    • 4
  • P. H. Schreier
    • 1
  1. 1.Bayer CropScience AGMonheimGermany
  2. 2.Department of Plant Breeding and Yield PhysiologyMax Planck Institute for Plant Breeding ResearchKölnGermany
  3. 3.Department of Organismic InteractionsMax Planck Institute for Terrestrial MicrobiologyMarburgGermany
  4. 4.Department of BiologyPhilipps-Universität MarburgMarburgGermany
  5. 5.Direvo Biotech AGNattermannallee 1KölnGermany
  6. 6.Roche Diagnostics GmbHPenzbergGermany