Molecular Genetics and Genomics

, Volume 267, Issue 6, pp 814–819

Cloning of AFLP markers linked to resistance to Peronosclerospora sorghi in maize

Authors

  •  H. Agrama
    • Gulf Coast Research and Education Center, University of Florida, 5007 60th Street East, Bradenton, FL 34203-9511, USA
  •  S. Houssin
    • Agricultural Research Center, Geiza, Egypt
  •  M. Tarek
    • Agricultural Research Center, Geiza, Egypt
Original Paper

DOI: 10.1007/s00438-002-0713-2

Cite this article as:
Agrama, H., Houssin, S. & Tarek, M. Mol Gen Genomics (2002) 267: 814. doi:10.1007/s00438-002-0713-2
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Abstract.

Genetic mapping of resistance genes for sorghum downy mildew (SDM) in maize revealed multiple-locus inheritance. A combination of AFLP (amplified fragment length polymorphism) technique with bulked segregant analysis (BSA) was applied to map the genes involved in the resistance to SDM (Peronosclerospora sorghi) in a recombinant inbred population. Three AFLP markers were identified and mapped to chromosomes 1 and 9, in regions previously associated with SDM resistance. One other AFLP marker was found to be associated with disease susceptibility but could not be linked to any chromosome. These four AFLP fragments were isolated, cloned and sequenced. A BLAST search of the GenBank database showed that none of these four sequences was closely related to resistance genes that have been reported previously. Sequence-characterized amplified regions (SCARs) were produced and used to assess the presence of SDM resistance genes and characterize specific genotypes. These markers may be useful in marker-assisted breeding programs.

Bulked segregant analysis DNA sequence Sequence-characterized amplified region (SCAR) Sorghum downy mildew Resistance gene
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© Springer-Verlag 2002