Molecular Genetics and Genomics

, Volume 267, Issue 2, pp 218–222

Cloning of a prolidase gene from Aspergillus nidulans and characterisation of its product

Authors

  •  R. Jalving
    • Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
  •  P. Bron
    • Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
  •  H. Kester
    • Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
  •  J. Visser
    • Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
  •  P. Schaap
    • Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands
Original Paper

DOI: 10.1007/s00438-002-0655-8

Cite this article as:
Jalving, R., Bron, P., Kester, H. et al. Mol Gen Genomics (2002) 267: 218. doi:10.1007/s00438-002-0655-8

Abstract.

Using EST sequence information available from the filamentous fungus Aspergillus nidulans as a starting point, we have cloned the prolidase-encoding gene, designated pepP. Introduction of multiple copies of this gene into the A. nidulans genome leads to overexpression of an intracellular prolidase activity. Prolidase was subsequently purified and characterised from an overexpressing strain. The enzyme activity is dependent on manganese as a cofactor, is specific for dipeptides and hydrolyses only dipeptides with a C-terminal proline residue. Although these proline dipeptides are released both intracellularly and extracellularly, prolidase activity was detected only intracellularly.

Metallopeptidase Dipeptidase Filamentous fungus Prolidase

Copyright information

© Springer-Verlag 2002