Molecular Genetics and Genomics

, Volume 266, Issue 5, pp 740–746

Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA

  •  Y. Ogihara
  •  K. Isono
  •  T. Kojima
  •  A. Endo
  •  M. Hanaoka
  •  T. Shiina
  •  T. Terachi
  •  S. Utsugi
  •  M. Murata
  •  N. Mori
  •  S. Takumi
  •  K. Ikeo
  •  T. Gojobori
  •  R. Murai
  •  K. Murai
  •  Y. Matsuoka
  •  Y. Ohnishi
  •  H. Tajiri
  •  K. Tsunewaki
Original Paper

DOI: 10.1007/s00438-001-0606-9

Cite this article as:
Ogihara, Y., Isono, K., Kojima, T. et al. Mol Gen Genomics (2002) 266: 740. doi:10.1007/s00438-001-0606-9

Abstract.

Structural features of the wheat plastome were clarified by comparison of the complete sequence of wheat chloroplast DNA with those of rice and maize chloroplast genomes. The wheat plastome consists of a 134,545-bp circular molecule with 20,703-bp inverted repeats and the same gene content as the rice and maize plastomes. However, some structural divergence was found even in the coding regions of genes. These alterations are due to illegitimate recombination between two short direct repeats and/or replication slippage. Overall comparison of chloroplast DNAs among the three cereals indicated the presence of some hot-spot regions for length mutations. Whereas the region with clustered tRNA genes and that downstream of rbcL showed divergence in a species-specific manner, the deletion patterns of ORFs in the inverted-repeat regions and the borders between the inverted repeats and the small single-copy region support the notion that wheat and rice are related more closely to each other than to maize.

Chinese Spring wheat Chloroplast DNA Complete sequence Hypervariable region Structure analysis

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Y. Ogihara
    • 1
  •  K. Isono
    • 2
  •  T. Kojima
    • 1
  •  A. Endo
    • 1
  •  M. Hanaoka
    • 3
  •  T. Shiina
    • 3
  •  T. Terachi
    • 4
  •  S. Utsugi
    • 5
  •  M. Murata
    • 5
  •  N. Mori
    • 6
  •  S. Takumi
    • 6
  •  K. Ikeo
    • 7
  •  T. Gojobori
    • 7
  •  R. Murai
    • 8
  •  K. Murai
    • 8
  •  Y. Matsuoka
    • 8
  •  Y. Ohnishi
    • 8
  •  H. Tajiri
    • 8
  •  K. Tsunewaki
    • 8
  1. 1.Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Yokohama 244-0813, Japan
  2. 2.PE Biosystems Japan Ltd, Chiba 279-0011, Japan
  3. 3.Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan
  4. 4.Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kyoto 603-8047, Japan
  5. 5.Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan
  6. 6.Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University, Kobe 657-8501, Japan
  7. 7.Center for Information Biology, National Institute of Genetics, Mishima 411-8540, Japan
  8. 8.Department of Bioscience, Fukui Prefectural University, Fukui 910-1195, Japan