Molecular Genetics and Genomics

, Volume 266, Issue 5, pp 832–837

A role for HNF-3 in the regulation of the HNF-1 gene of the Atlantic salmon

  •  C. Stenson-Cox
  •  A. McNair
  •  M. Curley
  •  T. Smith
  •  F. Gannon
Original Paper

DOI: 10.1007/s00438-001-0602-0

Cite this article as:
Stenson-Cox, C., McNair, A., Curley, M. et al. Mol Gen Genomics (2002) 266: 832. doi:10.1007/s00438-001-0602-0

Abstract.

Hepatocyte nuclear factors -1 (HNF-1) and -3 (HNF-3) are hepatocyte-enriched transcription factors that are central to the establishment and maintenance of the liver phenotype in vertebrates. In the present study we demonstrate that, in the Atlantic salmon, asHNF-3 regulates the expression of the gene for asHNF-1. Multiple putative binding sites for asHNF-3 were identified within the 5′ flanking region of the HNF-1 gene using a computer-based algorithm, and these were confirmed to be functional by electrophoretic mobility shift assays. In transient transfection assays it was shown that co-expression of asHNF-3 leads to a decrease in the promoter activity of the 5′ flanking region of the asHNF-1 gene.

Hepatic Nuclear Factor-1/-3 Transcriptional network Salmo salar 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  C. Stenson-Cox
    • 1
  •  A. McNair
    • 3
  •  M. Curley
    • 1
  •  T. Smith
    • 1
  •  F. Gannon
    • 4
  1. 1.National Diagnostics Centre/BioResearch Ireland, National University of Ireland, Galway, Ireland
  2. 2.National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland
  3. 3.Centre de Biotechnologie Moléculaire, UNIL-EPFL, 1015 Lausanne, Switzerland
  4. 4.European Molecular Biology Laboratory (EMBL), Postfach 1022, Meyerhofstrasse, 69117, Heidelberg, Germany