Parasitology Research

, Volume 84, Issue 8, pp 657–667

Echinococcus multilocularis: relationship between susceptibility/ resistance and liver fibrogenesis in experimental mice

  • Sylviane Guerret
  • Dominique A. Vuitton
  • Martine Liance
  • Clotilde Pater
  • Jean Pierre Carbillet
ORIGINAL PAPER

DOI: 10.1007/s004360050466

Cite this article as:
Guerret, S., Vuitton, D., Liance, M. et al. Parasitol Res (1998) 84: 657. doi:10.1007/s004360050466

Abstract

To analyze collagen and other matrix protein deposits in experimental alveolar echinococcosis as well as the expression of lysyl oxidase, the enzyme that initiates the first steps in the pyridinoline cross-linking of collagen, and to establish a relationship between resistance/susceptibility to Echinococcus multilocularis larval growth and fibrogenesis, we compared AKR/J mice (susceptible to E. multilocularis infection) with NMRI mice (resistant hosts) in this study. Collagen deposits in the lesions were evaluated using a colorimetric method; the nature of matrix proteins involved in the periparasitic fibrosis and lysyl oxidase expression were assessed using immunostaining on tissue sections. The results obtained in this sequential study confirm that fibrogenesis is an important aspect of the host immune reaction against parasitic development and that both the extent and the course of matrix protein deposition differ in the liver of susceptible and resistant mice, respectively. The long-lasting expression of α-actin and lysyl oxidase by host cells in NMRI mice suggests that in this resistant strain, fibrosis was not only more developed but also more highly cross-linked and, thus, less sensitive to collagenases than in susceptible mice. A very strong expression of lysyl oxidase by parasitic cells was observed in both strains of mice; the observation that E. multilocularis itself has a role in lysyl oxidase cross-linking of host collagens can be hypothesized and would be a new example of parasite-host interplay.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Sylviane Guerret
    • 1
  • Dominique A. Vuitton
    • 2
  • Martine Liance
    • 3
  • Clotilde Pater
    • 2
  • Jean Pierre Carbillet
    • 2
  1. 1.Laboratoire de Pathologie Cellulaire du foie, Institut Pasteur, Lyon, FranceFR
  2. 2.WHO Collaborating Center for Prevention and Treatment of Human Echinococcosis, SERF Unit, University of Franche-Comté, Besançon, FranceFR
  3. 3.Department of Parasitology, University Paris-Val de Marne, Créteil, FranceFR